{"id":85950,"date":"2018-03-10T00:10:29","date_gmt":"2018-03-10T00:10:29","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/determinantes-qua%c2%admicos-y-moleculares-del-los-sitio-s-de-union-de-anestesicos-locales-en-canales-de-potasio-clonados-de-ventra%c2%adculo-humano-hkv-15\/"},"modified":"2018-03-10T00:10:29","modified_gmt":"2018-03-10T00:10:29","slug":"determinantes-qua%c2%admicos-y-moleculares-del-los-sitio-s-de-union-de-anestesicos-locales-en-canales-de-potasio-clonados-de-ventra%c2%adculo-humano-hkv-15","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-medicas\/determinantes-qua%c2%admicos-y-moleculares-del-los-sitio-s-de-union-de-anestesicos-locales-en-canales-de-potasio-clonados-de-ventra%c2%adculo-humano-hkv-15\/","title":{"rendered":"Determinantes qu\u00edmicos y moleculares del\/los sitio\/s de uni\u00f3n de anest\u00e9sicos locales en canales de potasio clonados de ventr\u00edculo humano (hkv 1,5)"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Monica Longobardo Martin <\/strong><\/h2>\n<p>La presente tesis doctoral estudia los determinantes qu\u00edmicos y moleculares de la estereoselectividad de los sitios de uni\u00f3n de anest\u00e9sicos locales en un canal de potasio clonado de ventr\u00edculo humano perteneciente a la famila shaker (hkv1,5). Para la realizaci\u00f3n de este estudio analizamos los efectos de los enanti\u00f3meros de anest\u00e9sicos locales pertenecientes al grupo de las pipecoloxilidades sobre la corriente i\u00f3nica generada tras la activaci\u00f3nd e canales de potasio hkv1,5. Expresados de forma estable en una l\u00ednea celular de fibroblastos de rat\u00f3n (ltk).  para ello, utilizamos la configuraci\u00f3n de c\u00e9lula enterea de la t\u00e9cnica de fijaci\u00f3n de voltaje en parches de membrana (\u00abpatch-clamp\u00bb). Asimismo, estudiamos los efectos de los enanti\u00f3meros de la bupivaca\u00edna y de un derivado cuaternario de la misma en canales hkv1,5 nativos y condistintas mutaciones dirigidas. Los resultados obtenidos demuestran que: 1 los anest\u00e9sicos locales del tipo de la pipecoloxilidida (bupivaca\u00edna, ropivaca\u00edna y mepivaca\u00edna) se unen a un recptor localizado en la cara interna del canal hkv1,5 y accesible cuando \u00e9ste se encuentra en estado abiero, 2 el sustituyente enposici\u00f3n 1 representa un determinante crucial d ela potencia y del grado de estereoselectividad inducido por este grupo de f\u00e1rmacaso, 3 la estereoselectividad del bloque inducido por lso enanti\u00f3meros de la bupivaca\u00edna requiere una interacci\u00f3n polar conla treonina enposici\u00f3n 505 del canal (t505) y, al menos, dos interacciones hidrof\u00f3bicas con la leucina y la valina presentes enposiciones 508 y 512 del canal hkv1,5 (l508 y v512).  estos resultados indican que el receptor para la bupvica\u00edna localizado en la cara interna del canal hkv1,5 est\u00e1 formado, al menos, por estos tres amino\u00e1cidos: t505,l508 y v512. Finalmente, los resultados de los estudios realizados conun an\u00e1logo permanentemente cargado de la bupivaca\u00edna indican que, 4 los canales hkv1,5 presentan, adem\u00e1s del receptor<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Determinantes qu\u00edmicos y moleculares del\/los sitio\/s de uni\u00f3n de anest\u00e9sicos locales en canales de potasio clonados de ventr\u00edculo humano (hkv 1,5)<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Determinantes qu\u00edmicos y moleculares del\/los sitio\/s de uni\u00f3n de anest\u00e9sicos locales en canales de potasio clonados de ventr\u00edculo humano (hkv 1,5) <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Monica Longobardo Martin <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 14\/07\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Carmen Valenzuela Miranda<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan  Luis Tamargo men\u00e9ndez <\/li>\n<li>valent\u00edn Ce\u00f1a callejo (vocal)<\/li>\n<li>Jes\u00fas Florez beledo (vocal)<\/li>\n<li>Juan  Manuel Duarte p\u00e9rez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Monica Longobardo Martin La presente tesis doctoral estudia los determinantes qu\u00edmicos y moleculares de la estereoselectividad de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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