{"id":86304,"date":"2000-12-09T00:00:00","date_gmt":"2000-12-09T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/modificacion-genetica-de-microorganismos-para-el-desamargado-de-zumos-citricos\/"},"modified":"2000-12-09T00:00:00","modified_gmt":"2000-12-09T00:00:00","slug":"modificacion-genetica-de-microorganismos-para-el-desamargado-de-zumos-citricos","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/modificacion-genetica-de-microorganismos-para-el-desamargado-de-zumos-citricos\/","title":{"rendered":"Modificacion genetica de microorganismos para el desamargado de zumos citricos"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Lesher Gordillo Julia M. <\/strong><\/h2>\n<p>El amargos debido a limonoides es un f\u00e9nomeno conocido como amargor retardado y se presenta en los zumos c\u00edtricos despu\u00e9s de que el zumo ha sido extra\u00eddo.  se han encontrado enzimas implicadas en el metabolismo de los limonoides que tienen la capacidad desamargar zumos c\u00edtricos. As\u00ed como microorganismos que pueden expresar constitutivamente estas enzimas, como es el caso de rhondococcus fascians, un abacteria gram positiva, que sin embargo no puede ser utilizada inmovilizada en reactores ya que no est\u00e1 descrita como un organismo gras por ser pat\u00f3genas de plantas.  por lo que en eata tesis se desarrolla diversas t\u00e9cnicas de biolog\u00eda molecular para llevar a cabo el aislamiento del gen 1 dh de rhodococcus fascians. las t\u00e9cnicas utilizadas estuvieron basadas principalmente en pcr, como pcr inversa, pcr con adaptadores, y pcr previa digesti\u00f3n parcial, que permitieron el aislamiento de algunas secuencias que podr\u00edan tratarse del gen 1dh, una de ellas presenta una homolog\u00eda del 70% a nivel de dna con el gen rv2578c de mycobacterium tuberculosis, y al traducir esta secuencia a p\u00e9ptido la homolog\u00eda aumenta al 80%, adem\u00e1s de presentar una alta significaci\u00f3n biol\u00f3gica al analizar filogen\u00e9ticamente ambas especies.  tambi\u00e9n ha sido posible el dise\u00f1o de obligonucle\u00f3tidos espec\u00edficos a partir de dos secuencias obtenidas por pdpcr, que permitieron el desarrollo de un \u00abgene walking\u00bb mediante \u00abvectoreta\u00bb.  por otra parte se han iniciado estudios de expresi\u00f3n diferencial, utilizando c\u00e9lulas de rhodococcus fascians que presentan diferente actividad de la enzima limonoato deshidrogenasa.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Modificacion genetica de microorganismos para el desamargado de zumos citricos<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Modificacion genetica de microorganismos para el desamargado de zumos citricos <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Lesher Gordillo Julia M. <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 C\u00f3rdoba<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 12\/09\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Manuel Rold\u00e1n Nogueras<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jes\u00fas D\u00edez Dapena <\/li>\n<li>Mar\u00eda Piqueras Jose (vocal)<\/li>\n<li>Jos\u00e9 M\u00aa Maldonado Ruiz (vocal)<\/li>\n<li>Alejandra Duran Encarnacion (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Lesher Gordillo Julia M. El amargos debido a limonoides es un f\u00e9nomeno conocido como amargor retardado y [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[160,973,940,17],"tags":[181239,2150,34691,2154,181237,181238],"class_list":["post-86304","post","type-post","status-publish","format-standard","hentry","category-bioquimica","category-cordoba","category-genetica-bioquimica","category-quimica","tag-alejandra-duran-encarnacion","tag-jesus-diez-dapena","tag-jose-manuel-roldan-nogueras","tag-jose-ma-maldonado-ruiz","tag-lesher-gordillo-julia-m","tag-maria-piqueras-jose"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/86304","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=86304"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/86304\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=86304"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=86304"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=86304"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}