{"id":86649,"date":"2018-03-10T00:11:16","date_gmt":"2018-03-10T00:11:16","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/regulacion-del-factor-de-transcripcion-especifico-de-hipofisis-pit-1-por-peptidos-secretagogos-de-la-hormona-de-crecimiento\/"},"modified":"2018-03-10T00:11:16","modified_gmt":"2018-03-10T00:11:16","slug":"regulacion-del-factor-de-transcripcion-especifico-de-hipofisis-pit-1-por-peptidos-secretagogos-de-la-hormona-de-crecimiento","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/regulacion-del-factor-de-transcripcion-especifico-de-hipofisis-pit-1-por-peptidos-secretagogos-de-la-hormona-de-crecimiento\/","title":{"rendered":"Regulacion del factor de transcripcion especifico de hipofisis (pit-1) por peptidos secretagogos de la hormona de crecimiento."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Angel Garcia Alonso <\/strong><\/h2>\n<p>Los secretagogos de la hormona de crecimiento ghs son un conjunto de mol\u00e9culas sint\u00e9ticas de naturaleza pept\u00eddica y no pept\u00eddica. La clonaci\u00f3n del recptor para estos liberadores no cl\u00e1sicos de gh (hormona de crecimiento) conjuntamente con la m\u00e1s reciente caracterizaci\u00f3nde su ligando end\u00f3geno, llamado ghrelin, ha supuesto de forma enequ\u00edvoca la demostraci\u00f3n de la existencia de un nuevo sistema fisiol\u00f3gico que regula la secreci\u00f3n de gh.  la expresi\u00f3n del gen de gh depende de un factor de transcripci\u00f3nespc\u00edfico de la hip\u00f3fisis, llamado pit-1, el objetivo de este trabajo es determinar si el hexap\u00e9ptido sint\u00e9tico ghrp-6, un referente en el mundo de los ghs, as\u00ed como el ligando end\u00f3geno ghrelin regulan la expresi\u00f3nd el gen d epit-1,dado que el efecto de estos compuestos sobre la secreci\u00f3n de gh parece ser m\u00e1s prominente en ratas neonatas, se ensayaron los efectos de ghrp-6 sobre la expresi\u00f3n del gen pit-1 en cultivos monocapa de c\u00e9lulas adenohipofisarias (ah) de ratas macho de 15 d\u00edas, as\u00ed como los efectos sobre el promotor de gende pit-1 mediante el uso de una l\u00ednea celuar hek-293-ghs-r que sobreexpresa el receptor de los ghs (ghs-r). Finalmente se compararon los efectos de ghrelin y ghrp-6 sobre el promotor de pit-1.  mediante una combiaci\u00f3n de northern blot (estudio del mrna) y western blot (estudio a nivel de prot\u00ednas) se comprob\u00f3 que ghrp-6 activa de forma tiempo y dosis-dependiente la expresi\u00f3n del gen de pit-1 en cultivos de c\u00e9lulas ah de ratas macho de 15 d\u00edas. As\u00ed mismo se comprob\u00f1o que este efecto es directo bore el gen de pit-1, mientras que el efecto (tabmi\u00e9n observado) de ghrp-6 sobre la expresi\u00f3n de los genes de gh y prolactina pudiera ser mediado por el propio pit-1 as\u00ed mismo, mediante ria (radioinmunoensayo) se vio que ghrp-6 a una dosisi 10-6 m causaba un aumento en la gh secretada por las c\u00e9lulas al medio de cultivo tras 4h de tratamiento.  mediante experimentos de trasnfecci\u00f3n en la<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Regulacion del factor de transcripcion especifico de hipofisis (pit-1) por peptidos secretagogos de la hormona de crecimiento.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Regulacion del factor de transcripcion especifico de hipofisis (pit-1) por peptidos secretagogos de la hormona de crecimiento. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Angel Garcia Alonso <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Santiago de compostela<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 29\/09\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Carlos Dieguez Gonzalez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: manuel Freire rama <\/li>\n<li>Fernando Dominguez puente (vocal)<\/li>\n<li>Jos\u00e9 Castro perez (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Angel Garcia Alonso Los secretagogos de la hormona de crecimiento ghs son un conjunto de mol\u00e9culas sint\u00e9ticas [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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