{"id":86805,"date":"2000-06-10T00:00:00","date_gmt":"2000-06-10T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-de-la-regulacion-de-la-expresion-del-gen-pcbab-de-penicillium-chrysogenum-el-primer-gen-de-la-via-biosintetica-de-la-penicilina\/"},"modified":"2000-06-10T00:00:00","modified_gmt":"2000-06-10T00:00:00","slug":"analisis-de-la-regulacion-de-la-expresion-del-gen-pcbab-de-penicillium-chrysogenum-el-primer-gen-de-la-via-biosintetica-de-la-penicilina","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/analisis-de-la-regulacion-de-la-expresion-del-gen-pcbab-de-penicillium-chrysogenum-el-primer-gen-de-la-via-biosintetica-de-la-penicilina\/","title":{"rendered":"Analisis de la regulacion de la expresion del gen pcbab de penicillium chrysogenum, el primer gen de la via biosintetica de la penicilina"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Katarina Kosalkova <\/strong><\/h2>\n<p>La tesis doctoral se ha orientado al estudio de la expresi\u00f3n del gen pcbab de penicillum chrysogenum, el primer gen de la via biosintetica de la penicilina. Este gen codifica la enzima a-aminoadipil-cisteinil-valina sintetasa(acv sintetasa). La expresi\u00f3n de gen pcbab se considera el paso limitante de la biosintesis de penicilina. Se cree que en el control de la regulaci\u00f3n tanto de pcbab como de pcbc interviene la zona interg\u00e9nica situada entre ellos, que sirve como promotor bidireccional para ambos genes.  para determinar las regiones esenciales en la regulaci\u00f3n del gen pcbab se obtuvieron delecciones 5\u00c2\u00bfconsecutivas del promotor pcbab, las cuales se fusionan con el gen testigo lacz y se introdujeron en forma de una copia en el locus pyrg en p.Chrysogenum npe10 pyrg(mutante no productor de penicilina). el estudio de la expresi\u00f3n del gen pcbab, usando el gen la lacz como el gen testigo revelo la existencia de represi\u00f3n por glucosa en todos los transformantes que expresan el gen pcbab. Asimismo se han seleccionado tres regiones del promotor pcbab, denominadas cajas a,b y c, cuya delecci\u00f3n conduce a una significativa disminuci\u00f3n de la expresi\u00f3n del gen pcbab. se ha observado la formaci\u00f3n de complejos de uni\u00f3n al adn con las cajas a y b, usando los extractos preparados a partir de los micelios crecidos con glucosa y lactosa. En concreto el complejo ag1, usando la sonda a y los complejos bg1,bg2 y bl1, usando la sonda b. La secuencia de uni\u00f3n involucrada en la formaci\u00f3n del complejo ag1 es el heptanucleotido palindr\u00f3mico ttagtaa,situado en las posiciones -679 a -673 respecto al sitio de inicio de la transcripci\u00f3n del gen pcbab. La sustituci\u00f3n de una timina en la posici\u00f3n 1 \u00f3 5 en el heptanucleotido ttagtaa es suficiente para impedir la formaci\u00f3n del complejo ag1 seg\u00fan se observa en ensayos de alteraci\u00f3n de la movilidad electrofor\u00e9tica (emsa). El estudio de la expresi\u00f3n in vivo revela que la secuencia ttagtaa es imprescin<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Analisis de la regulacion de la expresion del gen pcbab de penicillium chrysogenum, el primer gen de la via biosintetica de la penicilina<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Analisis de la regulacion de la expresion del gen pcbab de penicillium chrysogenum, el primer gen de la via biosintetica de la penicilina <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Katarina Kosalkova <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Le\u00f3n<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 06\/10\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Francisco Mart\u00edn Mart\u00edn<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: fernando Moreno sanz <\/li>\n<li>teresa Suarez gonzalez (vocal)<\/li>\n<li>angel Duran bravo (vocal)<\/li>\n<li>Jos\u00e9 Luis Barredo fuente (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Katarina Kosalkova La tesis doctoral se ha orientado al estudio de la expresi\u00f3n del gen pcbab de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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