{"id":86852,"date":"2000-09-10T00:00:00","date_gmt":"2000-09-10T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/regulacion-por-insulina-de-los-enzimas-clave-de-la-via-glucolitica-gluconeogenica-en-cultivo-primario-de-hepatocitos-de-rata-obesa-zucker-fa-fa\/"},"modified":"2000-09-10T00:00:00","modified_gmt":"2000-09-10T00:00:00","slug":"regulacion-por-insulina-de-los-enzimas-clave-de-la-via-glucolitica-gluconeogenica-en-cultivo-primario-de-hepatocitos-de-rata-obesa-zucker-fa-fa","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/regulacion-por-insulina-de-los-enzimas-clave-de-la-via-glucolitica-gluconeogenica-en-cultivo-primario-de-hepatocitos-de-rata-obesa-zucker-fa-fa\/","title":{"rendered":"Regulacion por insulina de los enzimas clave de la via glucolitica\/gluconeogenica en cultivo primario de hepatocitos de rata obesa zucker (fa\/fa)."},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Carrillo De La Fuente Juan  Jos\u00e9 <\/strong><\/h2>\n<p>Los hepatocitos de rata en cultivo primario sufren un proceso de desdiferenciaci\u00f3n. por ello, en una primera etapa estudiamos el perfil fenot\u00edpico de la v\u00eda glucol\u00edtica\/gluconeog\u00e9nica en hepatocitos en cultivo. As\u00ed, observamos que se produc\u00eda una r\u00e1pida disminuci\u00f3n de los nivels de actividad y mrna de los enzimas clave de la v\u00eda gluconeog\u00e9ncia durante la evoluci\u00f3n del cultivo. estos cambios llevan a una marcada disminuci\u00f3n de la capacidad gluconeog\u00e9nica de los hepatocitos en cultivo en un plazo de 24 horas.  por otra parte se estudi\u00f3 la resitencia a insulina, tanto a corto como a largo plazo, en cultivo primario de hapatocitos de ratas obesas zucker (fa\/fa). Estos mantienen en cultivo primario su fenotipo caracter\u00edstico a nivel de la v\u00eda glucol\u00edtica\/gluconeog\u00e9ncia, durante al menos 24 horas. adem\u00e1s, presentan resistencia de la v\u00eda glucol\u00edtica\/gluconeog\u00e9ncia a la modulaci\u00f3n por sinsulina en sus acciona a corto plazo, fque se manifesta en ausencia de inhibici\u00f3n significativa por insulina de la gluconeog\u00e9nesis y de la producci\u00f3n hep\u00e1tica de glucosa (glucogenolisis m\u00e1s gluconeog\u00e9nesis), acompa\u00f1ada de una falta de activaci\u00f3n de la pfk-2 y ausencia de cambios en la concentraci\u00f3n de f-2,6-p2.  en cuanto a las acciones a largo plazo de la insulina, se observ\u00f3 resistencia a la modulaci\u00f3n de la actividad pepck y la expresi\u00f3n de su correspondiente mansajero.  por \u00faltimo, se estudiaron las v\u00edas de se\u00f1alizaci\u00f3n implicadas en la activaci\u00f3n a corto plazo de la pk-l por insulina. As\u00ed, se observ\u00f3 que en este proceso se requiere la activaci\u00f3n de las v\u00edas de se\u00f1aizaci\u00f3n de la p44\/p42 mapk y de la pi3-k. Adem\u00e1s, es posible que sea necesaria la activaci\u00f3n de la pkb, mientras que podemos descartar la participaci\u00f3n de la p70 s6-k.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Regulacion por insulina de los enzimas clave de la via glucolitica\/gluconeogenica en cultivo primario de hepatocitos de rata obesa zucker (fa\/fa).<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Regulacion por insulina de los enzimas clave de la via glucolitica\/gluconeogenica en cultivo primario de hepatocitos de rata obesa zucker (fa\/fa). <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Carrillo De La Fuente Juan  Jos\u00e9 <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 09\/10\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Emilio Feliu Albi\u00f1ana<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: enrique Bl\u00e1zquez fern\u00e1ndez <\/li>\n<li>ramon Bartrons bach (vocal)<\/li>\n<li>Antonio Sillero repullo (vocal)<\/li>\n<li>lisardo Bosc\u00e1 gomar (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Carrillo De La Fuente Juan Jos\u00e9 Los hepatocitos de rata en cultivo primario sufren un proceso de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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