{"id":87049,"date":"2018-03-10T00:11:47","date_gmt":"2018-03-10T00:11:47","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-de-los-genes-implicados-enla-biosa%c2%adntesis-del-antibiotico-a201a-en-streptomyces-capreolus-nrrl-3817\/"},"modified":"2018-03-10T00:11:47","modified_gmt":"2018-03-10T00:11:47","slug":"caracterizacion-de-los-genes-implicados-enla-biosa%c2%adntesis-del-antibiotico-a201a-en-streptomyces-capreolus-nrrl-3817","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/caracterizacion-de-los-genes-implicados-enla-biosa%c2%adntesis-del-antibiotico-a201a-en-streptomyces-capreolus-nrrl-3817\/","title":{"rendered":"Caracterizaci\u00f3n de los genes implicados enla bios\u00edntesis del antibi\u00f3tico a201a en streptomyces capreolus nrrl 3817"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Irene Saugar G\u00f3mez <\/strong><\/h2>\n<p>El antibi\u00f3tico nucleos\u00eddico a201a posee una estructura qu\u00edmica h\u00edbrida entre la del aminogluc\u00f3sido higromicina a y la del tambi\u00e9n nucle\u00f3sido puromicina. este antibi\u00f3tico es sintetizado por streptomyces capreolus nrrl 3817. Se han aislado a partir de una genoteca del mismo cuatro c\u00f3smidos, en cuyos insertos solapantes se podr\u00eda localizar el \u00abcluster\u00bb g\u00e9nico de bios\u00edntesis del antibi\u00f3tico y los determinantes de resistencia frente al mismo. El fragmento de adn cromos\u00f3mico mide aproximadamente 35 kb y su secuenciaci\u00f3n ha relevado la presencia de 28 posibles orfs. Los productos deducidos de dichas orfs se han comparado con las prote\u00ednas existentes en los bancos de datos y de esta manera, se ha elaborado una hipot\u00e9tica ruta de bios\u00edntesis del antibi\u00f3tico.  entre las orfs secuenciadas se han localizado hom\u00f3logos a los genes pur3, pur4, pur5, pur7 y pur10 del cluster pur para la bios\u00edntesis de puromicina en streptomyces alboniger. Disponemos en nuestro laboratorio de mutantes de delecci\u00f3n de s.Alboniger en los genes pur5, pur4 y pur10, defectivos en la producci\u00f3n de puromicina. Se ha conseguido restablecer la producci\u00f3n de puromicina en dichos mutantes mediante la complementaci\u00f3n en trans con sus respectivos genes hom\u00f3logs a2apur5, a2a-pur4 y a2apur10 respectivamente.  tambi\u00e9n se han tratado de determinar los posibles mecanismos de regulaci\u00f3n de la bios\u00edntesis de a201a, as\u00ed como de obtener un m\u00e9todo para la transformaci\u00f3n de s.Capreolus, pero en ninguno de los dos casosse han conseguido resultados positivos.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n de los genes implicados enla bios\u00edntesis del antibi\u00f3tico a201a en streptomyces capreolus nrrl 3817<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n de los genes implicados enla bios\u00edntesis del antibi\u00f3tico a201a en streptomyces capreolus nrrl 3817 <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Irene Saugar G\u00f3mez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 24\/10\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Martinez Jimenez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan  pedro Garc\u00eda ballesta <\/li>\n<li>Francisco Malpartida romero (vocal)<\/li>\n<li>Jos\u00e9 Antonio Salas fernandez (vocal)<\/li>\n<li> Pe\u00f1alva soto Miguel angel (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Irene Saugar G\u00f3mez El antibi\u00f3tico nucleos\u00eddico a201a posee una estructura qu\u00edmica h\u00edbrida entre la del aminogluc\u00f3sido higromicina [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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