{"id":87099,"date":"2018-03-10T00:11:51","date_gmt":"2018-03-10T00:11:51","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-de-las-modificaciones-co-y-post-traduccionales-de-la-poliproteina-del-virus-de-la-bursitis-infecciosa\/"},"modified":"2018-03-10T00:11:51","modified_gmt":"2018-03-10T00:11:51","slug":"caracterizacion-de-las-modificaciones-co-y-post-traduccionales-de-la-poliproteina-del-virus-de-la-bursitis-infecciosa","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/caracterizacion-de-las-modificaciones-co-y-post-traduccionales-de-la-poliproteina-del-virus-de-la-bursitis-infecciosa\/","title":{"rendered":"Caracterizaci\u00f3n de las modificaciones co y post-traduccionales de la poliproteina del virus de la bursitis infecciosa."},"content":{"rendered":"<h2>Tesis doctoral de <strong>  S\u00e1nchez Martinez Ana Belen <\/strong><\/h2>\n<p>El virus de la bursitis infecciosa (ibdv) es un virus icosa\u00e9drico de 60 nm de di\u00e1metro que carece de envuelta. Esta constituido por dos segmentos de arn bicatenario. El segmento a de 3,2 kpb contiene dos fases de lectura abierta. La orf1 codifica la prote\u00edna vp5 de 17kda. La segunda fase de lectura abierta orf2 codifica la poliprote\u00edna de 110kda que es procesada autocatal\u00edticamente dando lugar a las prote\u00ednas vpx, vp3 y vp4, siendo esta \u00faltima una lon-proteasa responsable del procesamiento. Posteriormente mediante procesamiento post-traduccional la prote\u00edna vpx genera la prote\u00edna vp2. El segmento b de 3kpb codifica la prote\u00edna vp1 de 90 kda que es la arn polimerasa dependiente de arn.  en la presente tesis se ha caracterizado en primer lugar los sitios de procesamiento en la poliprote\u00edna mediante mutag\u00e9nesis dirigida y posterior an\u00e1lisis por expresi\u00f3n transitoria. Se ha demostrado que las secuencias 511laa513 y 754maa756 son las dianas de procesamiento entre las regiones vpx-vp4 y vp4-vp3 485 aqaasgtaraasgkaraas503 contiene tres sitios secundarios de procesamiento en la regi\u00f3n vpx y vp4.  en busca de un mayor conocimiento y caracterizaci\u00f3n se analizaron las prote\u00ednas virales, mediante electroforesis bidimensional. Esta metodolog\u00eda nos ha permitido determinar la presencia de modificaciones post-traduccionales. se identificaron las prote\u00ednas vpx, vp4 y vp3 mediante autoradiograf\u00eda, tinci\u00f3n de plata y western-blot. As\u00ed se demostr\u00f3  la presencia de 4 isoformas mayoritarias de la prote\u00edna vp3. Para la detecci\u00f3n de las modificaciones responsables de la presencia de las diferentes isoformas de la prote\u00edna de vp3 se utilizaron las t\u00e9cnicas de espectrometr\u00eda de masas y nanoelectrospray. as\u00ed se pudo confirmar la dimetilaci\u00f3n de los residuos 827k y 832k en dos de las isoformas de la prote\u00edna vp3.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n de las modificaciones co y post-traduccionales de la poliproteina del virus de la bursitis infecciosa.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n de las modificaciones co y post-traduccionales de la poliproteina del virus de la bursitis infecciosa. <\/li>\n<li><strong>Autor:<\/strong>\u00a0  S\u00e1nchez Martinez Ana Belen <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 27\/10\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Rodr\u00edguez Aguirre Jos\u00e9 Francisco<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: julio Coll morales <\/li>\n<li>ignacio Casal (vocal)<\/li>\n<li>Jos\u00e9 Mar\u00eda Almendral del rio (vocal)<\/li>\n<li>amelia Nieto martin (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de S\u00e1nchez Martinez Ana Belen El virus de la bursitis infecciosa (ibdv) es un virus icosa\u00e9drico de 60 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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