{"id":87197,"date":"2000-03-11T00:00:00","date_gmt":"2000-03-11T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/expresion-en-vectores-eucariontes-de-genes-para-vacunacion-dna-por-inmersion-en-el-modelo-trucha-rabdo-virus\/"},"modified":"2000-03-11T00:00:00","modified_gmt":"2000-03-11T00:00:00","slug":"expresion-en-vectores-eucariontes-de-genes-para-vacunacion-dna-por-inmersion-en-el-modelo-trucha-rabdo-virus","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/expresion-en-vectores-eucariontes-de-genes-para-vacunacion-dna-por-inmersion-en-el-modelo-trucha-rabdo-virus\/","title":{"rendered":"Expresi\u00f3n en vectores eucariontes de genes para vacunaci\u00f3n dna por inmersi\u00f3n en el modelo trucha\/rabdo virus"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Mirian Fernandez Alonso <\/strong><\/h2>\n<p>Se ha estudiado la vacunaci\u00f3n dna por inmersi\u00f3n de truchas arco iris (onchorrynchus mykiss, walbaum) frente al virus de la septicimia hemorr\u00e1gica v\u00edrica (vshv). Se ha utilizado pl\u00e1smidos codificando la glicoprote\u00edna de membrnaa g, bajo los promotores de cytomegalovirus (cmv) y m\u00fasculo depex (fg2).  el trabajo se ha realizado primero a nivel celular utilizando ensayos de transfecci\u00f3n in vitro. Despu\u00e9s, con truchas inmunoimcompetentes ( 2 cm), utilizando ensayos de transfecci\u00f3n in vivo con pl\u00e1smidos que contienen los genes marcadores de la prote\u00edna verde fluorescente (gfp) y de la b-galactosidasa (b-gal).  por \u00faltimo, con truchas inmunocompetentes (5-7 cm), utilizando ensayos de vacunaci\u00f3n\/contraprueba con pl\u00e1smidos que codifican la prote\u00edna g, seguidos de estimaci\u00f3n de la respuesta humoral, celular y resistencia a la comtraprueba.  se ha seleccionado como potencialmente viables como m\u00e9todos de vacunaci\u00f3n por inmersi\u00f3n los ultrasonidos de baja frecuencia, los liposomas dotap y la bactofecci\u00f3n. La mayor duraci\u00f3n de la expresi\u00f3n de gfp en truchas inmunoincompetentes, los mayores niveles de inducci\u00f3n de anticuerpos anti-frg*11 de la prote\u00edna g de vshv en elisa y la mayor protecci\u00f3n (50%) de truchas inmunizadas con el gen de la g se han conseguido mediante vacunaci\u00f3n dna utilizando ultrasonidos de baja frecuencia. Su correlaci\u00f3n demuestra el valor predictivo  de \u00e9stos. Adem\u00e1s, se ha aportado evidencia experimental ara una posible sustituci\u00f3n  del promotor cmv por fg2, de importancia en la posible comercializaci\u00f3n de una vacuna.  la continuaci\u00f3n de estos estudios a peque\u00f1a y a gran escala podr\u00eda llevar al dise\u00f1o de un m\u00e9todo pr\u00e1ctico, econ\u00f3mico y efectivo de vacunaci\u00f3n de peces por inmersi\u00f3n.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Expresi\u00f3n en vectores eucariontes de genes para vacunaci\u00f3n dna por inmersi\u00f3n en el modelo trucha\/rabdo virus<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Expresi\u00f3n en vectores eucariontes de genes para vacunaci\u00f3n dna por inmersi\u00f3n en el modelo trucha\/rabdo virus <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Mirian Fernandez Alonso <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 03\/11\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Julio Coll Morales<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ram\u00f3n Luis D\u00edaz Garc\u00eda <\/li>\n<li>Manuel Rubio vallejo (vocal)<\/li>\n<li>Alberto Jos\u00e9 Villena cortes (vocal)<\/li>\n<li>Rafael Blasco lozano (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Mirian Fernandez Alonso Se ha estudiado la vacunaci\u00f3n dna por inmersi\u00f3n de truchas arco iris (onchorrynchus mykiss, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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