{"id":87714,"date":"2000-04-12T00:00:00","date_gmt":"2000-04-12T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/clonacion-de-genes-nutricionales-de-candida-albicans-y-su-utilizacion-como-herramientas-geneticas\/"},"modified":"2000-04-12T00:00:00","modified_gmt":"2000-04-12T00:00:00","slug":"clonacion-de-genes-nutricionales-de-candida-albicans-y-su-utilizacion-como-herramientas-geneticas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/clonacion-de-genes-nutricionales-de-candida-albicans-y-su-utilizacion-como-herramientas-geneticas\/","title":{"rendered":"Clonaci\u00f3n de genes nutricionales de candida albicans y su utilizaci\u00f3n como herramientas gen\u00e9ticas."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Ana Isabel Negredo Ant\u00f3n <\/strong><\/h2>\n<p>El aumento de la incidencia de las infecciones causadas por c. Albicans as\u00ed como la gravedad de estas en pacientes inmunocomprometidos ha hecho necesario abordar el car\u00e1cter pat\u00f3geno de esta levadura dim\u00f3rfica desde nuevos planteamientos, como son los que aporta la biolog\u00eda molecular y la gen\u00e9tica. Para ello se requiere desarrollar herramientas que permitan manipular gen\u00e9ticamente este microorganismo, trabajo que comenz\u00f3 a dar sus frutos en la d\u00e9cada de los a\u00f1os 80, sin embargo, hoy se siguen desarrollando y mejorando dichas herramientas por presentar c. Albicvans determinadas caracter\u00edsticas gen\u00e9ticas y que dificultan su manipulaci\u00f3n a este nivel. para la caracterizaci\u00f3n de los distintos procesos celulares es preciso, entre otras herramientas, construir cepas con marcadores gen\u00e9ticos obtenidas mediante interrupci\u00f3n g\u00e9nica as\u00ed como mejorar los vectores g\u00e9nicos.  se clonaron los genes nutricionales his4 y arg5,6 mediante complementaci\u00f3n, heter\u00f3loga y hom\u00f3loga respectivamente, de mutanes con deficiencias en estas rutas de bios\u00edntesis como posibles marcadores gen\u00e9ticos, y se utiliz\u00f3 el gen hisi con esta misma finalidad. Mediante la interrupci\u00f3n de los genes hisi y arg5,6 empleando el sistema de interrupci\u00f3n que permite recuperar el marcador de selecci\u00f3n(casete ura3) en la cepa cai4(ura3 )se obtubieron cepas con dos y tres marcadores gen\u00e9ticos, rm1000 (ura3 ,his1 ) y cnc43(ura3 ,his1 ,arg5,6 ). Estas dos cepas presentan una gran versatilidad, as\u00ed, la cepa rm100 posibilit\u00f3 desarrollar un nuevo m\u00e9todo de interrupci\u00f3n g\u00e9nica que consiste en interrumpir los dos alelos de un gen en un \u00fanico paso de transformaci\u00f3n empleando distintos marcadores para cada alelo. Por otra parte, la disponibilidad de la cepa cnc43 permiti\u00f3 desarrollar un sistema para demostrar la esencialidad de un gen en un microorganismo diploide como es c. Albicans.  el gen arg5,6 clonado se emple\u00f3 para la construcci\u00f3n dke una genoteca gen\u00f3mi<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Clonaci\u00f3n de genes nutricionales de candida albicans y su utilizaci\u00f3n como herramientas gen\u00e9ticas.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Clonaci\u00f3n de genes nutricionales de candida albicans y su utilizaci\u00f3n como herramientas gen\u00e9ticas. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Ana Isabel Negredo Ant\u00f3n <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 04\/12\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jes\u00fas Pla Alonso<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Mar\u00eda Molina mart\u00edn <\/li>\n<li>Rafael Sentandreu ramon (vocal)<\/li>\n<li>Miguel Sanchez  perez (vocal)<\/li>\n<li>germ\u00e1n Larriba calle (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Ana Isabel Negredo Ant\u00f3n El aumento de la incidencia de las infecciones causadas por c. Albicans as\u00ed [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[35,962,4578,278],"tags":[183453,2980,7554,24621,6618,9886],"class_list":["post-87714","post","type-post","status-publish","format-standard","hentry","category-ciencias-de-la-vida","category-genetica","category-ingenieria-genetica","category-microbiologia","tag-ana-isabel-negredo-anton","tag-german-larriba-calle","tag-jesus-pla-alonso","tag-maria-molina-martin","tag-miguel-sanchez-perez","tag-rafael-sentandreu-ramon"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/87714","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=87714"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/87714\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=87714"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=87714"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=87714"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}