{"id":87874,"date":"2018-03-10T00:12:46","date_gmt":"2018-03-10T00:12:46","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-comparativo-de-la-colagenasa-y-la-liberasa-en-la-obtencion-y-trasplante-de-islotes-pancreaticos-estudio-experimental\/"},"modified":"2018-03-10T00:12:46","modified_gmt":"2018-03-10T00:12:46","slug":"estudio-comparativo-de-la-colagenasa-y-la-liberasa-en-la-obtencion-y-trasplante-de-islotes-pancreaticos-estudio-experimental","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-medicas\/estudio-comparativo-de-la-colagenasa-y-la-liberasa-en-la-obtencion-y-trasplante-de-islotes-pancreaticos-estudio-experimental\/","title":{"rendered":"Estudio comparativo de la colagenasa y la liberasa en la obtenci\u00f3n y trasplante de islotes pancre\u00e1ticos. estudio experimental"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Francisco Garc\u00eda Cuy\u00e1s <\/strong><\/h2>\n<p>Introduccion  el transplante de islotes es un m\u00e9todo para restaurar la secreci\u00f3n de insulina en pacientes diab\u00e9ticos tipo i, pero no cumple las expectativas esperadas. Hasta el momento se ha empleado colagenasa -un enzima obtendio de c.Histoliticum- para aislar los islotes pancre\u00e1ticos; en previos estudios hemos detectado la presencia de altas concentraciones de endotoxina en las preparaciones de colagenasa.Recientemente se ha introducido el uso de una nueva combinaci\u00f3n de enzimas purificados libres e endotoxina, la liberasa, para la digesti\u00f3n pancre\u00e1tica.  objetivo  el objeto principal de nuestro trabaja ha sido comparar la funcionalida de los islotes trasplantados aislados con colagenasa o liberasa en un modelo experimental de transplante sing\u00e9nico en ratas lewis diab\u00e9ticas por administraci\u00f3n de dosis \u00fanica de extreptozotocina (stz).  material y m\u00e9todos  se obtuvieron islotes por digesti\u00f3 pancre\u00e1tica con colagenasa (grupo 1 n=9) o liberasa (grupo 2 n=9) grupos de 1500 islotes purificados en gradiente discontinuo de densidad (pureza del 70%) fueron transplantados bajo la c\u00e1psula renal izquierda. Se monitoriz\u00f3 el peso, la glicemia y los niveles de insulina cada 2 d\u00edas por punci\u00f3n de la cola y se obtuvo suero por punci\u00f3n intracard\u00edaca. El ri\u00f1\u00f3n que ubicaba el injerto fue extra\u00eddo y congelado para su an\u00e1lisis histol\u00f3gico pasados 90 d\u00edas (animales normoglic\u00e9micos) o bien en el momento de la recurrencia de la enfermedad (glicemia-20 mmol).  resultados  en el grupo i (colagenasa), los niveles de glucosa basal se normalizaron a las 24 horas en todos los animales (n=9); la normoglicemia se mantuvo durante los tres d\u00edas posteriores al transplante en dos ratas, durante los siete d\u00edas posteriores en 3 ratas y hasta el final  del seguimiento de 90 d\u00edas en las cuatro ratas restantes. El grupo ii (liberasa) los niveles de glucosa se normalizaron en 6 ratas solo durante las 24 horas posteriores al trasplante, tra<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio comparativo de la colagenasa y la liberasa en la obtenci\u00f3n y trasplante de islotes pancre\u00e1ticos. estudio experimental<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio comparativo de la colagenasa y la liberasa en la obtenci\u00f3n y trasplante de islotes pancre\u00e1ticos. estudio experimental <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Francisco Garc\u00eda Cuy\u00e1s <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 13\/12\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jaume Fern\u00e1ndez Llamazares Rodriguez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: manuel Armengol carrasco <\/li>\n<li>Marta Vives pi (vocal)<\/li>\n<li>manel Monreal bosch (vocal)<\/li>\n<li>joan Vi\u00f1as salas (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Francisco Garc\u00eda Cuy\u00e1s Introduccion el transplante de islotes es un m\u00e9todo para restaurar la secreci\u00f3n de insulina [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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