{"id":88931,"date":"2018-03-10T00:13:59","date_gmt":"2018-03-10T00:13:59","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-y-purificacion-de-un-principio-activo-de-origen-bacteriano-con-actividad-antitumoral\/"},"modified":"2018-03-10T00:13:59","modified_gmt":"2018-03-10T00:13:59","slug":"caracterizacion-y-purificacion-de-un-principio-activo-de-origen-bacteriano-con-actividad-antitumoral","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/caracterizacion-y-purificacion-de-un-principio-activo-de-origen-bacteriano-con-actividad-antitumoral\/","title":{"rendered":"Caracterizaci\u00f3n y purificaci\u00f3n de un principio activo de origen bacteriano con actividad antitumoral"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Beatriz Montaner Ramoneda <\/strong><\/h2>\n<p>En este trabajo se estudi\u00f3 el efecto del sobrenadante (cs-2170), obtenido del cultivo de serratia marcescens 2170, en la viabilidad de diferentes l\u00edneas celulares cancerosas de origen hematopoy\u00e9tico (jurkat, nso, hl-60, molt-4 y ramos), gastrointestinal (dld-1, sw-620 y hgt-1), mama (mcf-7) y epitelial (a431), as\u00ed como en l\u00edneas celulares no cancerosas (nih-3t3, swiss-3t3, nrk y mdck). A trav\u00e9s del ensayo del mtt se observ\u00f3 un descenso r\u00e1pido (4h) en el n\u00famero de c\u00e9lulas viables. Este efecto citot\u00f3xico se produc\u00eda a trav\u00e9s de la apoptosis, seg\u00fan pudimos determinar por la fragmentaci\u00f3n del dna en escalera, la tinci\u00f3n nuclear con hoechst 33342 y por an\u00e1lisis por facs de la externalizaci\u00f3n de los residuos fosfatidilserina de la membrana celular. Nosotros hemos purificado la mol\u00e9cula responsable de este efecto apopt\u00f3tico y determinado su estructura qu\u00edmica. Para ello utilizamos distintos mutantes de s.Marcescens (of, wf y 933), que lo eran en la v\u00eda de s\u00edntesis de la prodigiosina (pg), y de esta forma vinos que la pg estaba implicada en la inducci\u00f3n de apoptosis. Esta evidencia fue corroborada por el an\u00e1lisis espectrosc\u00f3pico de la pg aislada de s.Marcescens.  estos resultados indican que pg, un inmunosupresor, induce apoptosis en c\u00e9lulas cancerosas, pero no tiene un efecto t\u00f3xico en c\u00e9lulas no cancerosas, existiendo la posibilidad de su utilidad terap\u00e9utica como agente antineopl\u00e1sico.  el mecanismo de pg tambi\u00e9n fue estudiado. Pg induce apoptosis de forma independiente a p53 y bcl-2. Este efecto fue bloqueado utilizando el inhibidor de caspasas z-vad.Fmk, indicando que est\u00e1n implciadas las caspasas. Tambi\u00e9n vimos por western blot que se produc\u00eda la fragmentaci\u00f3n de la parp, lo cual sirvi\u00f3 tambi\u00e9n para determinar la actividad de las caspasas.  por \u00faltimo, mostramos que la activaci\u00f3n de la pkc es necesaria para la protecci\u00f3n de las c\u00e9lulas frente a la apoptosis inducida por pg y que el efecto anti-apop<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n y purificaci\u00f3n de un principio activo de origen bacteriano con actividad antitumoral<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n y purificaci\u00f3n de un principio activo de origen bacteriano con actividad antitumoral <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Beatriz Montaner Ramoneda <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 19\/02\/2001<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Ricardo P\u00e9rez Tom\u00e1s<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: jordi Domingo feliubalad\u00f3 <\/li>\n<li>Mar\u00eda  isabel Fabregat romero (vocal)<\/li>\n<li>ernest Giralt lledo (vocal)<\/li>\n<li> Madrid cuevas Juan  Francisco (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Beatriz Montaner Ramoneda En este trabajo se estudi\u00f3 el efecto del sobrenadante (cs-2170), obtenido del cultivo de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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