{"id":90039,"date":"2001-07-05T00:00:00","date_gmt":"2001-07-05T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-bioquimica-de-la-dena-topoisomerasa-de-tipo-ii-de-streptococcus-pneumoniae\/"},"modified":"2001-07-05T00:00:00","modified_gmt":"2001-07-05T00:00:00","slug":"caracterizacion-bioquimica-de-la-dena-topoisomerasa-de-tipo-ii-de-streptococcus-pneumoniae","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/caracterizacion-bioquimica-de-la-dena-topoisomerasa-de-tipo-ii-de-streptococcus-pneumoniae\/","title":{"rendered":"Caracterizacion bioquimica de la dena topoisomerasa de tipo ii de streptococcus pneumoniae"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Esteban Fernandez Moreira <\/strong><\/h2>\n<p>En espa\u00f1a hay un 50% de cepas de de s.Pneumoniae resistentes a penicilina. las fluoroquinolonas (fqs) son un grupo de antimicrobianos alternativos para el tratamiento de las infecciones causadas por esta bacteria. Los blancos de las fqs son las dna topoisomerasas de tipo ii (dna girasay topoisomerasa iv), enzimas esenciales para la viabilidad celular que controlan la topolog\u00eda del dna. Para determinar c\u00faal de estas enzimas es el blanco primario para estos compuestos, clonamos, superproducimos y purificamos por separado mediante cromatografia las subunidades gyra y gyrb de la dna girasa y parc y pare de la topoisomerasa iv. Reconstituimos las enzimas a partir de sus subuinades. Establecimos un ensayo de superenrrollamiento de dna mediado por girasa y otro de decatenaci\u00f3n de kdna mediado por topoisomerasa iv. probamos la potencia inhiboria de cuatro fqs representativas sobre estos ensayos. Estos experimentos permitieron determinar bioquimicamente que la topoisomerasa iv es el blanco primario de la fqs en s.Pneumoniae.  asimismo se clon\u00f3 superprodujo y purific\u00f3 mediante colas de histidinas el dominio amino terminal de gyra, el cual posee el sitio activo de la enzima en concentraci\u00f3n y pureza suficiente para su cristalizaci\u00f3n. Este dominio presento actividad de rotura de doble cadena en presencia de fqs cuando se acompleja con gyrb.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizacion bioquimica de la dena topoisomerasa de tipo ii de streptococcus pneumoniae<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizacion bioquimica de la dena topoisomerasa de tipo ii de streptococcus pneumoniae <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Esteban Fernandez Moreira <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 07\/05\/2001<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Gonzalez De La Campa Adela<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: manuel Espinosa padr\u00f3n <\/li>\n<li>Antonio Tormo garrido (vocal)<\/li>\n<li>Jos\u00e9 Berenguer Carlos (vocal)<\/li>\n<li>rosario Mu\u00f1oz moreno (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Esteban Fernandez Moreira En espa\u00f1a hay un 50% de cepas de de s.Pneumoniae resistentes a penicilina. las [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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