{"id":90920,"date":"2018-03-11T10:09:32","date_gmt":"2018-03-11T10:09:32","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/efecto-sobre-el-endotelio-linfatico-de-la-hipoxia-tumoral-e-inhibicion-de-la-produccion-de-vegf-por-flavonoides\/"},"modified":"2018-03-11T10:09:32","modified_gmt":"2018-03-11T10:09:32","slug":"efecto-sobre-el-endotelio-linfatico-de-la-hipoxia-tumoral-e-inhibicion-de-la-produccion-de-vegf-por-flavonoides","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/efecto-sobre-el-endotelio-linfatico-de-la-hipoxia-tumoral-e-inhibicion-de-la-produccion-de-vegf-por-flavonoides\/","title":{"rendered":"Efecto sobre el endotelio linf\u00e1tico de la hipoxia tumoral e inhibici\u00f3n de la producci\u00f3n de vegf por flavonoides"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Elena Ans\u00f3 Moncl\u00fas <\/strong><\/h2>\n<p>Vascular endothelial growth factor (vegf) is an important angiogenic and lymphangiogenic factor highly stimulated by hypoxia. We studied the effect of conditioned media obtained from h157 tumoral lung cancer cells incubated under normoxia (21% o2) or hypoxia (1% o2) on lymphatic endothelial cells (lecs). Gene expression was highly repressed in treated lecs and those related to cell adhesion and angiogenesis were highly perturbed. Functional assays concluded that hypoxic h157 conditioned medium induced lec proliferation, and cellular adhesion to collagens i and iv, but not to laminin or fibronectin. However, conditioned media did not affect to the adhesion tumoral cells to lecs. On the other hand, we studied the effect of flavonoids on the production of vegf stimulated by hypoxia. Fisetin, apigenin and luteolin were the most active compounds and hydroxyl substitutions at 3, 5, 7 and 4\u00c2\u00bf were important for inhibition of vegf production under hypoxic conditions. Moreover, our data showed that apoptotic flavonoids induced te phosphorylation of erk 1\/2. Finally, we demonstrated that the mechanism of action of these compounds involved a transcriptional regulation of vegf gene that were independent of hif- \u00e1 expression.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Efecto sobre el endotelio linf\u00e1tico de la hipoxia tumoral e inhibici\u00f3n de la producci\u00f3n de vegf por flavonoides<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Efecto sobre el endotelio linf\u00e1tico de la hipoxia tumoral e inhibici\u00f3n de la producci\u00f3n de vegf por flavonoides <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Elena Ans\u00f3 Moncl\u00fas <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 19\/12\/2008<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Jos\u00e9 Mart\u00ednez Irujo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Mar\u00eda  Jes\u00fas Lopez zabalza <\/li>\n<li>Mar\u00eda mercedes Garayoa berrueta (vocal)<\/li>\n<li>Mar\u00eda  isabel Marzo rubio (vocal)<\/li>\n<li>ignacio Jos\u00e9 Encio Martinez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Elena Ans\u00f3 Moncl\u00fas Vascular endothelial growth factor (vegf) is an important angiogenic and lymphangiogenic factor highly stimulated [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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