{"id":91188,"date":"2018-03-11T10:09:53","date_gmt":"2018-03-11T10:09:53","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/tipificacion-molecular-de-brucella-y-aplicacion-de-la-pcr-al-diagnostico-de-la-brucelosis\/"},"modified":"2018-03-11T10:09:53","modified_gmt":"2018-03-11T10:09:53","slug":"tipificacion-molecular-de-brucella-y-aplicacion-de-la-pcr-al-diagnostico-de-la-brucelosis","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/microbiologia\/tipificacion-molecular-de-brucella-y-aplicacion-de-la-pcr-al-diagnostico-de-la-brucelosis\/","title":{"rendered":"Tipificaci\u00f3n molecular de brucella y aplicaci\u00f3n de la pcr al diagn\u00f3stico de la brucelosis"},"content":{"rendered":"<h2>Tesis doctoral de <strong> David Garc\u00eda Yoldi <\/strong><\/h2>\n<p>Molecular typing of brucella y application of pcr for the diagnosis of brucellosis. david garc\u00eda yoldi. faculty of pharmacy, university of navarra (spain), 2009.  brucella causes brucellosis, one of the most important bacterial zoonosis in the world. The main objective was develop and evaluate different pcr-based techniques for the detection and molecular typing of brucella at species, biovar and strains levels. Different pcr-based techniques have been evaluated: conventional pcr, multiplex pcr, real time pcr, pcr-rflp and mlva (multiple-locus variable-number tandem repeat analysis). Genomic sequences of different brucella strains were analysed and conserved sequences were selected for the detection of genus brucella. In addition, based on the analysis of genetic polymorphisms among brucella species and biovars several molecular markers were selected (including  insertions, deletions, punctual mutations and tandem repeats). A new multiplex pcr assay (named bruce-ladder) was developed. This new test was specific for brucella and can identify and differentiate all the brucella species and the vaccine strains. Similarly, another multiplex pcr assay was able to discriminate among the five b. Suis biovars. A new taqman mgb real time pcr assays were also developed to detect brucella with a sensitivity of 100 fg of dna and a similar one step multiplex quantitative pcr assay can differentiate among b. Abortus, b. Melitensis, b. Suis and b. Ovis. The assessment of the genetic stability of the live attenuated vaccine b. Melitensis rev 1 was demonstrated by mlva analysis (mlva). However, mlva can demonstrate brucellosis outbreaks and to confirm that wildlife is a reservoir for zoonotic brucellosis in b. Suis strains. The results of this work have originated five published papers, one international patent and two commercial kits.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Tipificaci\u00f3n molecular de brucella y aplicaci\u00f3n de la pcr al diagn\u00f3stico de la brucelosis<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Tipificaci\u00f3n molecular de brucella y aplicaci\u00f3n de la pcr al diagn\u00f3stico de la brucelosis <\/li>\n<li><strong>Autor:<\/strong>\u00a0 David Garc\u00eda Yoldi <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 16\/01\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Ignacio Lopez Go\u00f1i<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan Garc\u00eda lobo <\/li>\n<li>clara Mar\u00eda Mar\u00edn alcala (vocal)<\/li>\n<li>Jos\u00e9 Luis Vizmanos p\u00e9rez (vocal)<\/li>\n<li>nieves Vizcaino santiso (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de David Garc\u00eda Yoldi Molecular typing of brucella y application of pcr for the diagnosis of brucellosis. david [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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