{"id":91382,"date":"2018-03-11T10:10:07","date_gmt":"2018-03-11T10:10:07","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/engineering-carbohydrate-active-enzymes-specificity-and-activity-remodeled\/"},"modified":"2018-03-11T10:10:07","modified_gmt":"2018-03-11T10:10:07","slug":"engineering-carbohydrate-active-enzymes-specificity-and-activity-remodeled","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/engineering-carbohydrate-active-enzymes-specificity-and-activity-remodeled\/","title":{"rendered":"Engineering carbohydrate-active enzymes: specificity and activity remodeled"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Trevor Addington <\/strong><\/h2>\n<p>Con la finalidad de entender y modificar la pared celular secundaria de las plantas, se fund\u00f3 el grupo enzyme discovery in hibrid aspen for fibern engineering (eden) compuesto por nueve laboratorios con la financiaci\u00f3n de la comisi\u00f3n europea. El principal objetivo de la b\u00fasqueda del grupo eden es ingeniar gen\u00e9ticamente la estructura de fibras para producir \u00e1rboles transg\u00e9nicos con propiedades modificadas para las industrias de la pulpa y el papel.  en el marco de este proyecto, se seleccion\u00f3 el populus tremula x tremuloides xilogluc\u00e1n endotransglicosilasa (pttxet16a) para estudiar en profundidad su actividad transglicosilasa catalizando la rotura y la reconnexi\u00f3n de mol\u00e9culas de xilogluc\u00e1n, el cual parece estar involucrado en la morfog\u00e9nesis de la pared celular secundaria. De esta forma, se intent\u00f3 crear una familia 16 de h\u00edbridos de la enzima activa con carbohidratos -glucanasa\/xet con la finalidad de dise\u00f1ar una enzima quim\u00e9rica con una o m\u00e1s de las propiedades siguientes alteradas: especificidad, actividad y\/o estabilidad.  las dos enzimas, bacillus licheniformis 1,3-1,4&#8211;glucanasa y populus tremula x tremuloides xilogluc\u00ed\u00a0 endotransglicosilasa, son miembros de la misma familia enzim\u00e1tica y tienen una gran homolog\u00eda en sus estructuras en 3-dimensiones. A\u00fan as\u00ed, estas enzimas presentan diferentes actividades, una tiene actividad hidrolasa y la otra, transferasa; diferentes especificidades, una acepta s\u00f3lo sustratos glicos\u00edlicos lineales mientras la otra, sustratos ramificados; y diferentes estabilidades.  la construcci\u00f3n de una enzima h\u00edbrida representa un reto dentro de la investigaci\u00f3n con la finalidad de entender qu\u00e9 caracter\u00edsticas f\u00edsicas de las dos enzimas se atribuyen al modelo espec\u00edfico de la actividad y especificidad observada. La extracci\u00f3n del lazo m\u00e1s grande de la 1,3-1,4&#8211;glucanasa result\u00f3 en la obtenci\u00f3n de una prote\u00edna plegada que todav\u00eda mantiene cierta actividad hidrolasa del gluc\u00e1n. A\u00fan as\u00ed, no se observ\u00f3 actividad o especificidad similar a la xilogluc\u00e1n endotransglicosilasa. A partir de este punto, se realizaron mutaciones puntuales a diferentes puntos de las hojas que forman la brecha del lugar de uni\u00f3n de la enzima por asemejarse a los residuos del pttxet16a. La prote\u00edna quim\u00e9rica final tampoco presentaba actividad xet ni -glucanasa. El an\u00e1lisis de la estructura por cristalograf\u00eda de rayos x revel\u00f3 una mayor reorganizaci\u00f3n estructural de la esperada proveyendo la nueva enzima de un claro espacio interno que obre muchas m\u00e1s puertas a la ingenier\u00eda de la enzima.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Engineering carbohydrate-active enzymes: specificity and activity remodeled<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Engineering carbohydrate-active enzymes: specificity and activity remodeled <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Trevor Addington <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Ram\u00f3n llull<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 26\/01\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Antonio Planas Sauter<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ignacio Fita rodriguez <\/li>\n<li>v\u00edctor Manuel Gonz\u00e1lez Miguel (vocal)<\/li>\n<li>francesc Canals sur\u00eds (vocal)<\/li>\n<li>olga m. Durany turk (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Trevor Addington Con la finalidad de entender y modificar la pared celular secundaria de las plantas, se [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[160,115,955,13696,32959],"tags":[32768,124038,15733,69374,189333,50597],"class_list":["post-91382","post","type-post","status-publish","format-standard","hentry","category-bioquimica","category-bioquimica-molecular","category-enzimologia","category-glucidos","category-ramon-llull","tag-antonio-planas-sauter","tag-francesc-canals-suris","tag-ignacio-fita-rodriguez","tag-olga-m-durany-turk","tag-trevor-addington","tag-victor-manuel-gonzalez-miguel"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/91382","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=91382"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/91382\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=91382"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=91382"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=91382"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}