{"id":93065,"date":"2018-03-11T10:12:18","date_gmt":"2018-03-11T10:12:18","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/structural-analysis-of-intracellular-transport-nuclear-entry-and-egress-of-herpes-simplex-virus-1-by-cryo-electron-tomography\/"},"modified":"2018-03-11T10:12:18","modified_gmt":"2018-03-11T10:12:18","slug":"structural-analysis-of-intracellular-transport-nuclear-entry-and-egress-of-herpes-simplex-virus-1-by-cryo-electron-tomography","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-molecular\/structural-analysis-of-intracellular-transport-nuclear-entry-and-egress-of-herpes-simplex-virus-1-by-cryo-electron-tomography\/","title":{"rendered":"Structural analysis of intracellular transport, nuclear entry and egress of herpes simplex virus 1 by cryo electron tomography."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Iosune Ibiricu Urriza <\/strong><\/h2>\n<p>Herpes simplex virus 1 (hsv\u00c2\u00bf1) is a complex enveloped double stranded dna virus. The capsid containing the dna is surrounded by a thick layer of tegument proteins and a lipid envelope with surface glycoproteins. In this work, cryo\u00c2\u00bfelectron tomography (cryo\u00c2\u00bfet), in combination with fluorescence microscopy, was applied to structurally analyze different stages of the hsv\u00c2\u00bf1 `life\u00c2\u00bf cycle in situ under close\u00c2\u00bfto native conditions and without any chemical fixation. The focus of this study was on intracellular viral transport, attachment of capsids to nuclear pores and viral egress from cells. after entry into host cells by membrane fusion, hsv\u00c2\u00bf1 capsids are devoid of most of their tegument and preserve the so\u00c2\u00bfcalled inner tegument. Capsids are then retrogradely transported towards the nucleus along microtubules, most probable by the cellular molecular motor complex dynein\u00c2\u00bfdynactin. After replication, progeny capsids exit the nucleus and are anterogradely transported towards the cell membrane, most likely by the cellular molecular motor kinesin\u00c2\u00bf1. At the final assembly site of the mature virus (virion) the capsids encounter membranous compartments that are associated with some of the tegument and viral glycoproteins. As a result, virions are further transported towards the cell membrane inside these compartments to exit the cell. transport of capsids was structurally analyzed in vivo and in vitro by cryo\u00c2\u00bfet and subtomogram averaging. Some of the inner tegument proteins are assumed to be essential for motor complex binding to the capsid during transport. However, the specific location of the inner tegument proteins and the attachment site of the motor complex have remained in dispute. Here it was revealed that the inner tegument was localized at the capsid vertices, binding to pentons and peripentonal hexons. This suggested that the motor complex binds only at the capsid vertices. surprisingly, not only capsids containing dna were transported in vivo, but also capsids devoid of dna. Significant differences in tegumentation could be observed between these two groups. an in vitro system was established to study the attachment of incoming capsids to nuclear pores. extracted xenopus laevis oocyte nuclei that were incubated with isolated capsids were spread and vitrified for cryo\u00c2\u00bfet observations. Several co\u00c2\u00bffactors that potentially influence the attachment (cytosol, importin\u00c2\u00bf\u00edY and atp) were tested. secondary envelopment, assembly and egress are the least understood processes in the hsv\u00c2\u00bf1 `life\u00c2\u00bf cycle. Cryo\u00c2\u00bfet of infected neurons provided new insights on the molecular structure of these events. An implication of clathrin during secondary envelopment and exit was revealed, as well as a specific orientation of the viral particles against the exit site. The formation of l\u00c2\u00bfparticles (noninfective viral particles produced during infection) was also characterized and showed many similarities to the egress pathway in virions. the structural analysis of virus\u00c2\u00bfhost cell interactions in vivo and in vitro provided new relevant biological insights into the hsv\u00c2\u00bf1 `life\u00c2\u00bf cycle, complementing existing biochemical data.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Structural analysis of intracellular transport, nuclear entry and egress of herpes simplex virus 1 by cryo electron tomography.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Structural analysis of intracellular transport, nuclear entry and egress of herpes simplex virus 1 by cryo electron tomography. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Iosune Ibiricu Urriza <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 29\/04\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Wolfgang Baumeister<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 Antonio Melero fondevilla <\/li>\n<li>juha Huiskonen (vocal)<\/li>\n<li>Jos\u00e9 Ruiz cast\u00f3n (vocal)<\/li>\n<li>christoph Hagen (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Iosune Ibiricu Urriza Herpes simplex virus 1 (hsv\u00c2\u00bf1) is a complex enveloped double stranded dna virus. The [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36483],"tags":[192317,192313,14396,192316,192315,192314],"class_list":["post-93065","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular","tag-christoph-hagen","tag-iosune-ibiricu-urriza","tag-jose-antonio-melero-fondevilla","tag-jose-ruiz-caston","tag-juha-huiskonen","tag-wolfgang-baumeister"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/93065","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=93065"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/93065\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=93065"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=93065"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=93065"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}