{"id":93338,"date":"2018-03-11T10:12:39","date_gmt":"2018-03-11T10:12:39","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/el-virus-de-la-enfermedad-de-newcastle-modelo-de-interaccion-virus-celula-y-vector-de-expresion\/"},"modified":"2018-03-11T10:12:39","modified_gmt":"2018-03-11T10:12:39","slug":"el-virus-de-la-enfermedad-de-newcastle-modelo-de-interaccion-virus-celula-y-vector-de-expresion","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/salamanca\/el-virus-de-la-enfermedad-de-newcastle-modelo-de-interaccion-virus-celula-y-vector-de-expresion\/","title":{"rendered":"El virus de la enfermedad de newcastle: modelo de interacci\u00f3n virus-c\u00e9lula y vector de expresi\u00f3n"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Juan Ayllon Barasoain <\/strong><\/h2>\n<p>El virus de la enfermedad de newcastle (ndv) es un virus aviar con genoma rna(-) no segmentado, perteneciente al g\u00e9nero avulavirus dentro de la familia paramyxoviridae. Utilizando t\u00e9cnicas de gen\u00e9tica inversa, en el presente trabajo se han rescatado ndv recombinantes (rndv) portando el gen de una glicoprote\u00edna transmembrana humana: la l-endoglina, un correceptor del tgf-beta implicado en numerosos fen\u00f3menos fisiol\u00f3gicos y fisiopatol\u00f3gicos. Las c\u00e9lulas infectadas por los rndv expresan altos niveles de endoglina y \u00e9sta se incorpora a las membranas de los viriones de la progenie, por lo que postulamos el uso de los rndv para sobreexpresar la prote\u00edna con vistas a su posterior purificaci\u00f3n. Los rndv con el gen de la endoglina pueden, adem\u00e1s, infectar cultivos primarios de c\u00e9lulas mesangiales humanas y reproducir en ellos la sobreexpresi\u00f3n de endoglina que sucede de forma natural en determinadas patolog\u00edas fibr\u00f3ticas renales. Proponemos que la infecci\u00f3n con los rndv puede generar modelos celulares para el estudio de este tipo de fen\u00f3menos.  en los virus con envoltura como el ndv, una fase fundamental en la interacci\u00f3n virus-c\u00e9lula es la fusi\u00f3n de membranas que permite el acceso del material gen\u00e9tico v\u00edrico a la c\u00e9lula infectada. Para estudiar este proceso se han mutado residuos en regiones clave de la prote\u00edna que lo hace posible, la prote\u00edna de fusi\u00f3n (f). Hemos analizado los niveles de expresi\u00f3n de los mutantes y su capacidad para promover la fusi\u00f3n c\u00e9lula-c\u00e9lula en distintas condiciones. Varias sustituciones aminoac\u00eddicas han producido prote\u00ednas hiperfusog\u00e9nicas indicando la importancia de los residuos mutados en la estabilidad y funcionamiento de la prote\u00edna f. Asimismo, dos de las sustituciones realizadas han conferido independencia respecto a la prote\u00edna de uni\u00f3n para inducir fusi\u00f3n, lo que es habitualmente un requisito estricto en los paramixovirus. Estas mutaciones podr\u00edan permitir la generaci\u00f3n de rndv m\u00e1s eficientes como vectores y como agentes oncol\u00edticos.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>El virus de la enfermedad de newcastle: modelo de interacci\u00f3n virus-c\u00e9lula y vector de expresi\u00f3n<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 El virus de la enfermedad de newcastle: modelo de interacci\u00f3n virus-c\u00e9lula y vector de expresi\u00f3n <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Juan Ayllon Barasoain <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Salamanca<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 16\/05\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Isabel Mu\u00f1oz  Barroso<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Mar\u00eda  angeles Serrano garcia <\/li>\n<li>Mar\u00eda  amparo Estepa perez (vocal)<\/li>\n<li>Rafael Blasco lozano (vocal)<\/li>\n<li>Jos\u00e9 Antonio Melero fondevilla (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Juan Ayllon Barasoain El virus de la enfermedad de newcastle (ndv) es un virus aviar con genoma [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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