{"id":93571,"date":"2018-03-11T10:12:58","date_gmt":"2018-03-11T10:12:58","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/utilizacion-de-fenol-por-rhodococcus-erythropolis-upv-1-clonacion-purificacion-y-caracterizacion-de-la-fenol-hidroxilasa\/"},"modified":"2018-03-11T10:12:58","modified_gmt":"2018-03-11T10:12:58","slug":"utilizacion-de-fenol-por-rhodococcus-erythropolis-upv-1-clonacion-purificacion-y-caracterizacion-de-la-fenol-hidroxilasa","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/enzimologia\/utilizacion-de-fenol-por-rhodococcus-erythropolis-upv-1-clonacion-purificacion-y-caracterizacion-de-la-fenol-hidroxilasa\/","title":{"rendered":"Utilizacion de fenol por rhodococcus erythropolis upv-1: clonacion, purificacion y caracterizacion de la fenol hidroxilasa"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Laura Saa Pe\u00f1a <\/strong><\/h2>\n<p>El fenol es un compuesto arom\u00e1tico que se encuentra a elevada concentraci\u00f3n en efluentes procedentes de distintas actividades industriales. Debido a los problemas medioambientales que se generan por su toxicidad, resulta de inter\u00e9s el estudio de su biodegradaci\u00f3n como herramienta para descontaminar los lugares afectados. R. Erythropolis upv-1 puede degradar eficientemente fenol y eliminar formaldeh\u00eddo de aguas residuales sint\u00e9ticas e industriales. En este trabajo se han estudiado etapas iniciales del catabolismo de fenol en esta bacteria, siendo estas la entrada de fenol al interior celular y su posterior hidroxilaci\u00f3n a catecol. Se ha detectado la existencia de un sistema de toma de fenol de elevada especificidad e inducible por dicho compuesto y se ha caracterizado su comportamiento respecto a algunos inhibidores del transporte. Adem\u00e1s, se ha identificado en el genoma de r. Erythropolis upv-1, los genes phea1 y phea2, responsables de la actividad fenol hidroxilasa. Ambos genes se han clonado, expresado en e. Coli y purificado como prote\u00ednas de fusi\u00f3n a colas de histidina. Las prote\u00ednas que codifican, his6phea1 e his6phea2 presentan similitud de secuencia con distintas monooxigenasas constituidas por dos componentes. Se ha caracterizado la actividad de ambas prote\u00ednas recombinantes, encontr\u00e1ndose que his6phea2 es una flavin reductasa que reduce fad a fadh2, el cual es empleado por his6phea1 para llevar a cabo la hidroxilaci\u00f3n del sustrato fen\u00f3lico.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Utilizacion de fenol por rhodococcus erythropolis upv-1: clonacion, purificacion y caracterizacion de la fenol hidroxilasa<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Utilizacion de fenol por rhodococcus erythropolis upv-1: clonacion, purificacion y caracterizacion de la fenol hidroxilasa <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Laura Saa Pe\u00f1a <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 28\/05\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Luis Serra Ferrer<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 Mar\u00eda Vega piqueres <\/li>\n<li>Mar\u00eda  auxiliadora Prieto jimenez (vocal)<\/li>\n<li>Francisco Castillo rodriguez (vocal)<\/li>\n<li>isabel Barcina lopez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Laura Saa Pe\u00f1a El fenol es un compuesto arom\u00e1tico que se encuentra a elevada concentraci\u00f3n en efluentes [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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