{"id":94124,"date":"2018-03-11T10:13:46","date_gmt":"2018-03-11T10:13:46","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/truncacion-de-gsk-3-por-calpaina-implicaciones-fisiologicas-y-patologicas\/"},"modified":"2018-03-11T10:13:46","modified_gmt":"2018-03-11T10:13:46","slug":"truncacion-de-gsk-3-por-calpaina-implicaciones-fisiologicas-y-patologicas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-molecular\/truncacion-de-gsk-3-por-calpaina-implicaciones-fisiologicas-y-patologicas\/","title":{"rendered":"Truncacion de gsk-3 por calpaina: implicaciones fisiologicas y patologicas"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Paloma Go\u00f1i Oliver <\/strong><\/h2>\n<p>Gsk-3 activity can be regulated by phosphorylation and through interaction with gsk-3-binding proteins; here we describe n-terminal proteolysis as a novel way to regulate gsk-3. Gsk-3\u00c2\u00bf and gsk-3\u00edY were truncated by calpain, generating two fragments of 40 and 30 kda, a proteolytic process that was inhibited by specific calpain inhibitors. The 30 kda fragment was originated by cleavage of the 49asp &#8211; 50arg peptide bound. These truncated isoforms were active kinases. We also found that lithium, a gsk-3 inhibitor, inhibits full-length and cleaved gsk-3 isoforms with the same ic50 value. When cultured cortical neurons were exposed with ionomycin, glutamate, or nmda, gsk-3 was truncated. This truncation was blocked by the calpain inhibitor calpeptin, at the same concentration at which it inhibits calpain-mediated cleavage of other well-known calpain substrates. Truncated gsk-3 forms did not accumulate, suggesting that a short-lived product is formed. Gsk-3 truncation mediated by calpain prevents its binding to 14-3-3\u00c2\u00bf. In addition, overexpression of the truncated gsk-3 form ([50-420]-gsk-3\u00edY) in cos-7 cells showed that the n-terminal is involved in nuclear import, since the truncated form is only present in the cytoplasm.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Truncacion de gsk-3 por calpaina: implicaciones fisiologicas y patologicas<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Truncacion de gsk-3 por calpaina: implicaciones fisiologicas y patologicas <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Paloma Go\u00f1i Oliver <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 17\/06\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Felix Hernandez Perez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: cecilio Gimenez martin <\/li>\n<li>Miguel Medina padilla (vocal)<\/li>\n<li>pilar Gomez ramos (vocal)<\/li>\n<li>teresa Iglesias vacas (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Paloma Go\u00f1i Oliver Gsk-3 activity can be regulated by phosphorylation and through interaction with gsk-3-binding proteins; here [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36483],"tags":[6730,93162,130036,194127,29976,66051],"class_list":["post-94124","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular","tag-cecilio-gimenez-martin","tag-felix-hernandez-perez","tag-miguel-medina-padilla","tag-paloma-goni-oliver","tag-pilar-gomez-ramos","tag-teresa-iglesias-vacas"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/94124","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=94124"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/94124\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=94124"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=94124"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=94124"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}