{"id":9432,"date":"1995-01-01T00:00:00","date_gmt":"1995-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/1995\/01\/01\/regulacion-de-la-sintesis-de-citocinas-por-pentoxifilina-y-buflomedil-en-cultivo-de-queratinocitos-estimulados-con-acetato-de-13-forbol-12-miristilo-tpa-y-acido-urocanico\/"},"modified":"1995-01-01T00:00:00","modified_gmt":"1995-01-01T00:00:00","slug":"regulacion-de-la-sintesis-de-citocinas-por-pentoxifilina-y-buflomedil-en-cultivo-de-queratinocitos-estimulados-con-acetato-de-13-forbol-12-miristilo-tpa-y-acido-urocanico","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-medicas\/regulacion-de-la-sintesis-de-citocinas-por-pentoxifilina-y-buflomedil-en-cultivo-de-queratinocitos-estimulados-con-acetato-de-13-forbol-12-miristilo-tpa-y-acido-urocanico\/","title":{"rendered":"Regulacion de la sintesis de citocinas por pentoxifilina y buflomedil en cultivo de queratinocitos estimulados con acetato de 13-forbol 12-miristilo (tpa) y acido urocanico."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Pedro Redondo Bellon <\/strong><\/h2>\n<p>Queratinocitos humanos procedentes de piel sana fueron cultivados durante largo tiempo en un medio libre de suero, y estimulados mediante 1ug\/ml de acetato de 13-forbol 12-miristilo (tpa) y acido urocanico (au) (10-100ug\/ml), que en su isomero cis es considerado un posible fotorreceptor cutaneo responsable de los fenomenos inflamatorios secundarios a radiacion uv-b.A nivel de arn (northern blot) y proteina (elisa), se midio la sintesis de las siguientes citocinas: tnf-x, il-1x, il-1b, il-4, il-6, il-8 y tgf-b1. Posteriormente se valoro la modulacion de esta sintesis mediante dos farmacos: la pentoxifilina (ptx) y el clorhidrato de buflomedil (cb) ambos a dosis de 100 y 1000ug\/ml.  el tpa aumento la expresion de arn de tnf-x, il-1-x, il-1-b y tgf-b-1. Se aprecio proteina activa de il-1-b en el sobrenadante de los cultivos en condiciones basales, sin modificarse sus niveles ni los de il-1-x- tras estimulo con tpa. No detectamos il-4 (arn ni proteina) y unicamente cantidades insignificantes de il-6 proteina.  obtuvimos concentraciones elevadas de il-8 en condiciones basales, que aumentaron tras estimulo con tpa.  el au en sus dos isomeros no indujo cambios en la sintesis proteica de las citocinas. Se aprecio una leve tendencia a aumentar la expresion de los genes de il-1x y tnf-x tras la administracion de 100ug\/ml de cis-au frente a trans-au y control.  tras estimulo con 1ug\/ml de tpa, la ptx y el cb mostraron una tendencia a disminuir la expresion de arn de il-1x e il-1b. La ptx no inhibio la transcripcion de tnf-x, y si ligeramente la sintesis proteica. El cb inhibio la sintesis de proteina de tnf-x e il-8.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Regulacion de la sintesis de citocinas por pentoxifilina y buflomedil en cultivo de queratinocitos estimulados con acetato de 13-forbol 12-miristilo (tpa) y acido urocanico.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Regulacion de la sintesis de citocinas por pentoxifilina y buflomedil en cultivo de queratinocitos estimulados con acetato de 13-forbol 12-miristilo (tpa) y acido urocanico. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Pedro Redondo Bellon <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1995<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Agustin Espa\u00f1a Alonso<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Santiago Santidrian Alegre <\/li>\n<li>Alfonso Sanchez Ibarrola (vocal)<\/li>\n<li>Jos\u00e9 Luis Diaz Perez (vocal)<\/li>\n<li>Carmen Herrero Mateu (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Pedro Redondo Bellon Queratinocitos humanos procedentes de piel sana fueron cultivados durante largo tiempo en un medio [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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