{"id":94578,"date":"2018-03-11T10:14:18","date_gmt":"2018-03-11T10:14:18","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-funcional-y-localizacion-de-receptores-muscara%c2%adnicos-en-la-cara-luminal-del-epitelio-colico-de-rata\/"},"modified":"2018-03-11T10:14:18","modified_gmt":"2018-03-11T10:14:18","slug":"caracterizacion-funcional-y-localizacion-de-receptores-muscara%c2%adnicos-en-la-cara-luminal-del-epitelio-colico-de-rata","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-funcional-y-localizacion-de-receptores-muscara%c2%adnicos-en-la-cara-luminal-del-epitelio-colico-de-rata\/","title":{"rendered":"Caracterizaci\u00f3n funcional y localizaci\u00f3n de receptores muscar\u00ednicos en la cara luminal del epitelio c\u00f3lico de rata"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Elena Milagrosa Molina Roldan <\/strong><\/h2>\n<p>Caracterizaci\u00f3n funcional y localizaci\u00f3n de receptores muscar\u00ednicos en la cara luminal del epitelio c\u00f3lico de rata. elena milagrosa molina rold\u00e1n .  servicio de medicina general y aparato digestivo i. Hospital cl\u00ednico san carlos. directores: prof. Dr . Jes\u00fas \u00e1lvarez fern\u00e1ndez-represa y dr. Julio mayol mart\u00ednez. departamento de cirug\u00eda. Facultad de medicina. U.C.M.  \tlos receptores de membrana acoplados a prote\u00ednas g (gpcr), y en concreto los receptores muscar\u00ednicos desempe\u00f1an un papel fundamental  en la regulaci\u00f3n funcional de las c\u00e9lulas epiteliales intestinales. El paradigma cl\u00e1sicamente aceptado sobre el modelo de secreci\u00f3n i\u00f3nica propone una distribuci\u00f3n asim\u00e9trica de los receptores muscar\u00ednicos, cuya expresi\u00f3n funcional est\u00e1 restringida a la membrana basolateral de las c\u00e9lulas epiteliales involucradas en la secreci\u00f3n de cloro. \trecientemente se ha demostrado la presencia de receptores muscar\u00ednicos tanto en la cara basolateral, como en la cara apical de c\u00e9lulas epiteliales de ri\u00f1\u00f3n. Estos experimentos plantearon la posibilidad de que tambi\u00e9n existieran receptores muscar\u00ednicos en la cara apical del epitelio c\u00f3lico, dada la similitud existente entre ambos tejidos. \tel objetivo de este trabajo es estudiar la presencia de receptores  muscar\u00ednicos en la cara apical del epitelio c\u00f3lico, mediante estudios electrofisiol\u00f3gicos y t\u00e9cnicas inmunohistoqu\u00edmicas. \tcon los experimentos electrofisiol\u00f3gicos se puede obtener, la corriente de cortocircuito (isc) que proporciona una medida del movimiento i\u00f3nico activo a trav\u00e9s de las membranas del epitelio. Estos estudios fueron realizados en ratas sprague-dawley macho. \u00e9stas se sacrificaron en una c\u00e1mara cerrada con exceso de co2; se extirparon los 5 cm distales de colon, manteni\u00e9ndolo en  isquemia fr\u00eda con una soluci\u00f3n de ringer, hasta el comienzo del experimento. Una vez montados en las c\u00e1maras ussing modificadas, se sustituy\u00f3 la soluci\u00f3n ringer fr\u00eda por soluci\u00f3n ringer a 37\u00c2\u00ba c, con oxigenaci\u00f3n contin\u00faa. Los procedimientos utilizado s en los experimentos electrofisiol\u00f3gicos se describen a continuaci\u00f3n: 1) se trataron las muestras del colon con carbacol (cch) 100 microm (an\u00e1logo a la acetilcolina) por la regi\u00f3n basolateral para compararlo con los controles no sometidos a tratamie nto. 2) se estudi\u00f3 la acci\u00f3n del cch 100 microm en el lado mucoso del colon para contrastarlo con los controles no tratados. 3) se trat\u00f3 las muestras del colon a\u00f1adiendo cch 100microm por la regi\u00f3n apical sumergidos en una soluci\u00f3n ringer sin cl- par<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n funcional y localizaci\u00f3n de receptores muscar\u00ednicos en la cara luminal del epitelio c\u00f3lico de rata<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n funcional y localizaci\u00f3n de receptores muscar\u00ednicos en la cara luminal del epitelio c\u00f3lico de rata <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Elena Milagrosa Molina Roldan <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 29\/06\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>represa \u00e1lvarez Fern\u00e1ndez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: d\u00e1maso S\u00e1nchez de vega Garc\u00eda <\/li>\n<li>Francisco Javier Padillo ruiz (vocal)<\/li>\n<li>Jos\u00e9 Antonio Rodr\u00edguez montes (vocal)<\/li>\n<li>Mar\u00eda -elena Mart\u00edn p\u00e9rez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Elena Milagrosa Molina Roldan Caracterizaci\u00f3n funcional y localizaci\u00f3n de receptores muscar\u00ednicos en la cara luminal del epitelio [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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