{"id":94711,"date":"2009-01-07T00:00:00","date_gmt":"2009-01-07T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/proteomica-en-drosophila-melanogaster\/"},"modified":"2009-01-07T00:00:00","modified_gmt":"2009-01-07T00:00:00","slug":"proteomica-en-drosophila-melanogaster","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-molecular\/proteomica-en-drosophila-melanogaster\/","title":{"rendered":"Prote\u00f3mica en drosophila melanogaster."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jana Alonso Lorenzo <\/strong><\/h2>\n<p>El disco imaginal de ala de drosophila melanogaster y conocer las prote\u00ednas implicadas en su desarrollo y morfog\u00e9nesis, son el objetivo principal de esta tesis.  La base de datos de prote\u00ednas de disco imaginal de ala se ha obtenido tras la utilizaci\u00f3n de dos t\u00e9cnicas anal\u00edticas: la electroforesis bidimensional de alta resoluci\u00f3n (2d-page) y la espectrometr\u00eda de masas (ms).  Para ello, se marcaron con 35[s]met+35[s]cys los polip\u00e9ptidos procedentes de la l\u00ednea celular schneider y se obtuvo su mapa bidimensional; el cual se compar\u00f3 con el mapa bidimensional del disco imaginal de ala.  Tras determinar las diferencias cualitativas y cuantitativas, se observo que ambos sistemas presentaban m\u00e1s de 600 polip\u00e9ptidos comunes.  Para la identificaci\u00f3n por espectrometr\u00eda de masas, se realizaron geles bidimensionales preparativos con una mezcla de polip\u00e9ptidos aislados a partir de c\u00e9lulas schneider y discos imaginales de ala marcados radiactivamente, y se analizaron mediante maldi-tof (matriz asisted laser desorption\/ionization \u00c2\u00bf time of flight) aquellos polip\u00e9ptidos comunes a ambos sistemas.  Mediante este abordaje experimental se identificaron 159 prote\u00ednas de la base de datos del disco imaginal de ala. asimismo, se caracterizaron el protema mitocondrial y ribosomal de drosophila, mediante el aislamiento y purificaci\u00f3n de dichos org\u00e1nulos a partir de larvas de tercer estadio y generando su mapa bidimensional mediante 2d-page.  La base de datos 2d mitocondrial conten\u00eda 231 polip\u00e9ptidos, y la ribosomal, 58. El an\u00e1lisis por maldi-tof, identific\u00f3 66 prote\u00ednas mitocondriales y 52 ribosomales de las bases de datos correspondientes. el objetivo principal de estos estudios es la identificaci\u00f3n de prote\u00ednas de drosophila cuyo nivel de expresi\u00f3n est\u00e9 aumentado o disminu\u00eddo entre los diferentes discos de larva de tercer estadio y\/o entre diferentes estadios durante su desarrollo.  Ese es el caso de ssp 6002, que se identific\u00f3 mediante huella pept\u00eddica como la prote\u00edna de choque t\u00e9rmico, heat shock protein 23 (hsp23).  La caracterizaci\u00f3n del patr\u00f3n de expresi\u00f3n durante el desarrollo mediante un anticuerpo generado contra la prote\u00edna nativa, revel\u00f3 su implicaci\u00f3n en la morfog\u00e9nesis del disco imaginal de ala. La expresi\u00f3n hsp23 est\u00e1 bajo el control del complejo as-c, conjunto de genes que especifican los territorios proneurales en el disco imaginal; y se mantiene inalterado en condiciones de choque t\u00e9rmico. tras estos resultados, extendimos el estudio a otra prote\u00edna de choque t\u00e9rmico, hsp60a, caracteriz\u00e1ndose su patr\u00f3n de expresi\u00f3n con un anticuerpo contra el polip\u00e9ptido nativo.  El an\u00e1lisis inmunohistoqu\u00edmico de discos imaginales y embriones, mostr\u00f3 la localizaci\u00f3n mitocondrial de hsp60a y un patr\u00f3n de expresi\u00f3n altamente din\u00e1mico durante la embriog\u00e9nesis, que se manten\u00eda en condiciones de estr\u00e9s t\u00e9rmico. La regulaci\u00f3n de la expresi\u00f3n de hsp60a se produc\u00eda a nivel post-transcripcional, aunque en situaciones de estr\u00e9s (apoptosis y altas temperaturas) su expresi\u00f3n se regula a nivel transcripcional en todo el embri\u00f3n.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Prote\u00f3mica en drosophila melanogaster.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Prote\u00f3mica en drosophila melanogaster. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jana Alonso Lorenzo <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/07\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan Fern\u00e1ndez Santar\u00e9n<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 manuel Cuezva marcos <\/li>\n<li>concepcion Gil garcia (vocal)<\/li>\n<li>angel Garcia alonso (vocal)<\/li>\n<li>Rafael Garesse alarc\u00f3n (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jana Alonso Lorenzo El disco imaginal de ala de drosophila melanogaster y conocer las prote\u00ednas implicadas en 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