{"id":95287,"date":"2018-03-11T10:15:15","date_gmt":"2018-03-11T10:15:15","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/funcion-de-la-proteica-orc4-en-la-iniciacion-de-la-replicacion-y-en-el-control-transcripcional-en-schizosaccharomyces-pombe\/"},"modified":"2018-03-11T10:15:15","modified_gmt":"2018-03-11T10:15:15","slug":"funcion-de-la-proteica-orc4-en-la-iniciacion-de-la-replicacion-y-en-el-control-transcripcional-en-schizosaccharomyces-pombe","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/levaduras\/funcion-de-la-proteica-orc4-en-la-iniciacion-de-la-replicacion-y-en-el-control-transcripcional-en-schizosaccharomyces-pombe\/","title":{"rendered":"Funci\u00f3n de la proteica orc4 en la iniciaci\u00f3n de la replicaci\u00f3n y en el control transcripcional en schizosaccharomyces pombe"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Laura Mojardin Menendez <\/strong><\/h2>\n<p>El complejo de reconocimiento del origen (orc) juega un importante papel en la iniciaci\u00f3n de la replicaci\u00f3n del dna en eucariotas. En schizosaccharomyces pombe, la uni\u00f3n de este complejo a los or\u00edgenes de replicaci\u00f3n in vitro e in vivo viene mediado por el extremo n-terminal de la subunidad orc4p. Este dominio contiene 9 motivos de uni\u00f3n al dna denominados at-hooks que son capaces de unirse a zonas ricas en adenina y timina sin requerimientos espec\u00edficos de secuencia. Para analizar en detalle las propiedades que permiten que orc4p se una al dna, hemos generado unas cepas en las que el gen orc4+ end\u00f3geno ha sido sustituido por una versi\u00f3n modificada con s\u00f3lo 3, 4, 5 \u00f3 7 at-hooks, pero que conserva el dominio c-terminal necesario para el correcto ensamblaje del complejo orc. El an\u00e1lisis por electroforesis bidimensional en geles de agarosa de varios or\u00edgenes de replicaci\u00f3n en estas cepas muestra que su eficiencia de activaci\u00f3n se ve disminuida por la presencia de una prote\u00edna orc4 mutante, probablemente como consecuencia de la menor afinidad por el dna que presentan los orc4 mutantes tal y como hemos comprobado por ensayos de inmunoprecipitaci\u00f3n de cromatina. Adem\u00e1s de estar implicado en la iniciaci\u00f3n de la replicaci\u00f3n, el complejo orc interviene en la represi\u00f3n transcripcional de regiones gen\u00f3micas en varios organismos. Para averiguar si este era el caso de s. Pombe comparamos el patr\u00f3n de expresi\u00f3n gen\u00f3mico del silvestre con el de las cepas mutantes de orc4. De esta manera, comprobamos que en las cepas mutantes se produc\u00eda una sobreexpresi\u00f3n de genes adyacentes a or\u00edgenes de replicaci\u00f3n y de las regiones heterocrom\u00e1ticas. Estos resultados sugieren un papel del complejo orc en la regulaci\u00f3n transcripcional de algunas regiones de la levadura de fisi\u00f3n.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Funci\u00f3n de la proteica orc4 en la iniciaci\u00f3n de la replicaci\u00f3n y en el control transcripcional en schizosaccharomyces pombe<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Funci\u00f3n de la proteica orc4 en la iniciaci\u00f3n de la replicaci\u00f3n y en el control transcripcional en schizosaccharomyces pombe <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Laura Mojardin Menendez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Salamanca<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 15\/07\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Francisco Antequera Marquez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: margarita Salas falgueras <\/li>\n<li>crisanto Gutierrez armenta (vocal)<\/li>\n<li>ferran Azorin marin (vocal)<\/li>\n<li>pablo Hern\u00e1ndez valenzuela (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Laura Mojardin Menendez El complejo de reconocimiento del origen (orc) juega un importante papel en la iniciaci\u00f3n [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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