{"id":95593,"date":"2018-03-11T10:15:36","date_gmt":"2018-03-11T10:15:36","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aislamiento-caracterizacion-y-plasticidad-de-las-celulas-madre-de-pulpa-dental\/"},"modified":"2018-03-11T10:15:36","modified_gmt":"2018-03-11T10:15:36","slug":"aislamiento-caracterizacion-y-plasticidad-de-las-celulas-madre-de-pulpa-dental","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/estomatologia-y-ortodoncia\/aislamiento-caracterizacion-y-plasticidad-de-las-celulas-madre-de-pulpa-dental\/","title":{"rendered":"Aislamiento, caracterizaci\u00f3n y plasticidad de las c\u00e9lulas madre de pulpa dental"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Francisco Javier Rodriguez Lozano <\/strong><\/h2>\n<p>Recientemente se ha descubierto la existencia de c\u00e9lulas madre en la pulpa dental (dpscs). Datos preliminares sugieren que estas c\u00e9lulas tienen capacidad de diferenciaci\u00f3n en osteoblastos, adipocitos y condrocitos. Si se confirman, estos datos apoyar\u00edan el uso de estas c\u00e9lulas, en la terapia celular de diferentes lesiones en odontolog\u00eda, abriendo una v\u00eda terap\u00e9utica completamente nueva. el objetivo de esta investigaci\u00f3n fue confirmar la existencia de c\u00e9lulas madre procedentes de la pulpa dental y realizar el aislamiento, caracterizaci\u00f3n y evaluaci\u00f3n de la capacidad pl\u00e1stica de estas c\u00e9lulas. para ello se extrajo pulpa dental de terceros molares humanos de pacientes j\u00f3venes (18-30 a\u00f1os), de la que se obtuvieron c\u00e9lulas que se colocaron en frascos de cultivo con un medio \u00f3ptimo para mantenimiento y expansi\u00f3n de c\u00e9lulas madre mesenquimales durante 3 semanas. Cuando se obtuvo la confluencia de c\u00e9lulas en los frascos, se cambi\u00f3 a un medio de cultivo inductor neural, osteobl\u00e1stico y adipog\u00e9nico durante un mes. La caracterizaci\u00f3n y diferenciaci\u00f3n de estas c\u00e9lulas se comprob\u00f3 mediante el uso de anticuerpos espec\u00edficos para el linaje mesenquimal y neural, tinciones como oil red y alizarin red, al igual que mediante las t\u00e9cnicas rt-pcr y western blott. las dpscs expresaron en estado basal los marcadores mesenquimales cd44, cd90, stro-1 cd105 y cd73 y los neurales bdnf, gdnf, nestina y ?-Iii- tubulina. Mediante pcr y western blott, se detectaron marcadores de origen embrionario y de cresta neural. Tras ser cultivadas con un medio neural, estas c\u00e9lulas sufr\u00edan cambios de morfolog\u00eda y expresaban o4, ng2, map1b, nestina y ?-Iii- tubulina. De igual modo que en presencia de un medio adipog\u00e9nico y osteobl\u00e1stico, formaron vacuolas lip\u00eddicas y dep\u00f3sitos c\u00e1lcicos tras un mes de inducci\u00f3n. nuestros resultados avalan la utilizaci\u00f3n de la pulpa dental como una nueva fuente de c\u00e9lulas madre adultas. Hemos desarrollado una tecnolog\u00eda que permite investigar si las dpscs pueden ser beneficiosas en terapia celular para, eventualmente, desarrollar ensayos cl\u00ednicos en patolog\u00eda odontol\u00f3gica y en enfermedades neurodegenerativas.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Aislamiento, caracterizaci\u00f3n y plasticidad de las c\u00e9lulas madre de pulpa dental<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Aislamiento, caracterizaci\u00f3n y plasticidad de las c\u00e9lulas madre de pulpa dental <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Francisco Javier Rodriguez Lozano <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 24\/07\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Salvador Mart\u00ednez P\u00e9rez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: joaquin Mar\u00eda Garcia-esta\u00f1 lopez <\/li>\n<li>augusto Silva gonzalez (vocal)<\/li>\n<li>Manuel joaquin De nova garcia (vocal)<\/li>\n<li>m. rosario Saez yuguero (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Francisco Javier Rodriguez Lozano Recientemente se ha descubierto la existencia de c\u00e9lulas madre en la pulpa dental [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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