{"id":95866,"date":"2009-11-09T00:00:00","date_gmt":"2009-11-09T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/structural-studies-of-membrane-proteins-the-coupling-protein-trwb-of-plasmid-r388-and-the-pore-forming-toxin-frac\/"},"modified":"2009-11-09T00:00:00","modified_gmt":"2009-11-09T00:00:00","slug":"structural-studies-of-membrane-proteins-the-coupling-protein-trwb-of-plasmid-r388-and-the-pore-forming-toxin-frac","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/estructura-molecular\/structural-studies-of-membrane-proteins-the-coupling-protein-trwb-of-plasmid-r388-and-the-pore-forming-toxin-frac\/","title":{"rendered":"Structural studies of membrane proteins: the coupling protein trwb of plasmid r388 and the pore-forming toxin frac"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Ariel Mechaly <\/strong><\/h2>\n<p>Las prote\u00ednas de membrana participan en una amplia variedad de procesos celulares tales como la comunicaci\u00f3n de c\u00e9lula a c\u00e9lula, el transporte de solutos y la conversi\u00f3n de energ\u00eda. Esta diversidad funcional se refleja en el gran n\u00famero de genes que codifican prote\u00ednas de membrana, por lo general, 20-30% de los proteomas de la mayor\u00eda de los organismos. Alrededor del 40% de las dianas terap\u00e9uticas son prote\u00ednas de membrana y por lo tanto conocer la estructura tridimensional de este tipo de macromol\u00e9culas ha despertado gran inter\u00e9s. Sin embargo, el n\u00famero disponible de estructuras de prote\u00ednas de membrana a alta resoluci\u00f3n es escaso. En este trabajo se realiz\u00f3 la caracterizaci\u00f3n estructural de dos prote\u00ednas membranas: la prote\u00edna acopladora trwb del pl\u00e1smido conjugativo r388 de e. Coli y la toxina formadora de poro frac de la an\u00e9mona de mar actinia fragacea. Utilizando dispersi\u00f3n de rayos-x a bajo \u00e1ngulo (saxs) se obtuvieron modelos a baja resoluci\u00f3n de trwb, estos modelos son consistentes con la estructura cristalogr\u00e1fica de trwb\u00c2\u00bfn70 correspondiente a su regi\u00f3n citos\u00f3lica y compatible con un dominio transmembrana formado por un manojo de doce \u00c2\u00bf-h\u00e9lices. Por cristalograf\u00eda de rayos-x se resolvi\u00f3 a 1.8 \u00ed\u00a5 de resoluci\u00f3n la estructura cuaternaria de la toxina formadora de poro frac perteneciente a la familia de las actinoporinas. La estructura nonam\u00e9rica resuelta corresponde a un intermediario no-l\u00edtico del mecanismo de formaci\u00f3n de poro por este tipo de toxinas.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Structural studies of membrane proteins: the coupling protein trwb of plasmid r388 and the pore-forming toxin frac<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Structural studies of membrane proteins: the coupling protein trwb of plasmid r388 and the pore-forming toxin frac <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Ariel Mechaly <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 11\/09\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Diego Marcelo A. Guerin Aguilar<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: felix Mar\u00eda Go\u00f1i urcelay <\/li>\n<li>pedro Alzari (vocal)<\/li>\n<li>hugo Monaco (vocal)<\/li>\n<li>armando Albert de la cruz (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Ariel Mechaly Las prote\u00ednas de membrana participan en una amplia variedad de procesos celulares tales como la [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1271,12909],"tags":[197050,14264,193273,12081,197051,95060],"class_list":["post-95866","post","type-post","status-publish","format-standard","hentry","category-estructura-molecular","category-pais-vasco-euskal-herriko-unibertsitatea","tag-ariel-mechaly","tag-armando-albert-de-la-cruz","tag-diego-marcelo-a-guerin-aguilar","tag-felix-maria-goni-urcelay","tag-hugo-monaco","tag-pedro-alzari"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/95866","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=95866"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/95866\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=95866"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=95866"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=95866"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}