{"id":95983,"date":"2018-03-11T10:16:08","date_gmt":"2018-03-11T10:16:08","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-bioqua%c2%admica-y-resolucion-de-la-estructura-tridimensional-de-una-nueva-d-2-hidroxiacido-deshidrogenasa-del-archaeon-haloferax-mediterranei\/"},"modified":"2018-03-11T10:16:08","modified_gmt":"2018-03-11T10:16:08","slug":"caracterizacion-bioqua%c2%admica-y-resolucion-de-la-estructura-tridimensional-de-una-nueva-d-2-hidroxiacido-deshidrogenasa-del-archaeon-haloferax-mediterranei","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/caracterizacion-bioqua%c2%admica-y-resolucion-de-la-estructura-tridimensional-de-una-nueva-d-2-hidroxiacido-deshidrogenasa-del-archaeon-haloferax-mediterranei\/","title":{"rendered":"Caracterizaci\u00f3n bioqu\u00edmica y resoluci\u00f3n de la estructura tridimensional de una nueva d-2-hidroxiacido deshidrogenasa del archaeon haloferax mediterranei"},"content":{"rendered":"<h2>Tesis doctoral de <strong> J\u00e9ssica Domenech P\u00e9rez <\/strong><\/h2>\n<p>El objetivo principal de la tesis es la obtenci\u00f3n y caracterizaci\u00f3n tanto bioqu\u00edmica como estructural de una nueva d-2-hidroxi\u00e1cido deshidrogenasa del haloarchaeon haloferax mediterranei. El estudio estructural de la prote\u00edna nos permitir\u00e1 entender la cat\u00e1lisis en la que se fundamenta la estereoespecificidad selectiva que presenta y la gran variedad de sustratos sobre los que act\u00faa. Para conseguir este objetivo ha sido necesario realizar el aislamiento del gen, el an\u00e1lisis funcional de la secuencia, la clonaci\u00f3n y expresi\u00f3n heter\u00f3loga del gen en escherichia coli, la caracterizaci\u00f3n bioqu\u00edmica de la prote\u00edna recombinante, la cristalizaci\u00f3n y la resoluci\u00f3n y an\u00e1lisis de la estructura tridimensional de la prote\u00edna. como resultado se ha obtenido una nueva d-2-hidroxi\u00e1cido deshidrogenasa que presenta especificidad dual de coenzima, siendo la primera enzima descrita perteneciente a esta familia con dicha caracter\u00edstica. Esta enzima presenta los rasgos propios de enzimas sintetizadas por organismos hal\u00f3filos como son un incremento en el contenido de residuos \u00e1cidos, una disminuci\u00f3n en el n\u00famero de lisinas y  elevada estabilidad a altas concentraciones salinas y a temperaturas mayores que sus contrapartidas mesof\u00edlicas. As\u00ed mismo, ha sido posible la obtenci\u00f3n de cristales de la prote\u00edna de elevada calidad que nos han permitido la determinaci\u00f3n de la estructura tridimensional con una resoluci\u00f3n de 1.35 a, siendo la mayor resoluci\u00f3n alcanzada hasta la fecha entre las prote\u00ednas halof\u00edlicas con estructura determinada. Gracias a ello hemos podido alcanzar los objetivos propuestos inicialmente.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n bioqu\u00edmica y resoluci\u00f3n de la estructura tridimensional de una nueva d-2-hidroxiacido deshidrogenasa del archaeon haloferax mediterranei<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n bioqu\u00edmica y resoluci\u00f3n de la estructura tridimensional de una nueva d-2-hidroxiacido deshidrogenasa del archaeon haloferax mediterranei <\/li>\n<li><strong>Autor:<\/strong>\u00a0 J\u00e9ssica Domenech P\u00e9rez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Alicante<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 18\/09\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan Ferrer Casanova<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: emilio San fabian maroto <\/li>\n<li>david william Rice (vocal)<\/li>\n<li>Jos\u00e9 Manuel Guis\u00e1n seijas (vocal)<\/li>\n<li>patrick Juli\u00e1n Baker (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de J\u00e9ssica Domenech P\u00e9rez El objetivo principal de la tesis es la obtenci\u00f3n y caracterizaci\u00f3n tanto bioqu\u00edmica como [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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