{"id":96010,"date":"2018-03-11T10:16:08","date_gmt":"2018-03-11T10:16:08","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/calcificaciones-vasculares-mecanismos-celulares-y-moleculares\/"},"modified":"2018-03-11T10:16:08","modified_gmt":"2018-03-11T10:16:08","slug":"calcificaciones-vasculares-mecanismos-celulares-y-moleculares","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/medicina-interna\/calcificaciones-vasculares-mecanismos-celulares-y-moleculares\/","title":{"rendered":"Calcificaciones vasculares: mecanismos celulares y moleculares"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Addy Rosa Montes De Oca Gonzalez <\/strong><\/h2>\n<p>La hiperfosfatemia est\u00e1 estrechamente ligada a las calcificaciones vasculares en la insuficiencia renal cr\u00f3nica. Asimismo, cuando son cultivadas con concentraciones altas de f\u00f3sforo (p), las c\u00e9lulas de m\u00fasculo liso vascular (cmlv) calcifican y experimentan una transdiferenciaci\u00f3n hacia un fenotipo osteog\u00e9nico. Dicha transici\u00f3n implica la disminuci\u00f3n de la expresi\u00f3n de marcadores miog\u00e9nicos, como sm22\u00c2\u00bf, y el aumento de prote\u00ednas caracter\u00edsticas de c\u00e9lulas osteog\u00e9nicas, como cbfa-1. Uno de los mecanismos epigen\u00e9ticos que ocasionan la disminuci\u00f3n en la expresi\u00f3n de ciertos genes es el silenciamiento g\u00e9nico mediante la metilaci\u00f3n de regiones reguladoras. Nuestra hip\u00f3tesis es que este mecanismo podr\u00eda estar involucrado en la transici\u00f3n osteog\u00e9nica de las cmlv. El objetivo general de esta tesis es el estudio de los mecanismos por los cuales el p induce cv en cmlv in vitro. Para desarrollarlo se estudiaron los siguientes aspectos en cmlv expuestas a alto p: el efecto del p en la calcificaci\u00f3n de las cmlv, as\u00ed como en la expresi\u00f3n de sm22\u00c2\u00bf y cbfa1; la metilaci\u00f3n del promotor de sm22\u00c2\u00bf; las consecuencias de la inhibici\u00f3n del cotransportador de p, pit-1; el potencial desmetilante de la proca\u00edna y el efecto de la desmetilaci\u00f3n en la cv y la expresi\u00f3n de sm22\u00c2\u00bf y cbfa1. Resultados: las cmlv cultivadas con alto p calcificaron y presentaron una disminuci\u00f3n en la expresi\u00f3n de sm22\u00c2\u00bf aunada al aumento en la expresi\u00f3n de cbfa1. Adem\u00e1s, en cmlv expuestas a alto p, el promotor de sm22\u00c2\u00bf result\u00f3 metilado de una manera tiempo-dependiente. La inhibici\u00f3n del pit-1 evit\u00f3 la calcificaci\u00f3n y la transdiferenciaci\u00f3n de cmlv, y tambi\u00e9n previno la metilaci\u00f3n del promotor de sm22\u00c2\u00bf. Adem\u00e1s, la adici\u00f3n de un agente desmetilante (proca\u00edna) al medio de cultivo con alto p revirti\u00f3 la metilaci\u00f3n del promotor de sm22\u00c2\u00bf, restableciendo la expresi\u00f3n del este gen, y previniendo la calcificaci\u00f3n y la expresi\u00f3n de cbfa1. Conclusi\u00f3n: la metilaci\u00f3n del promotor de sm22\u00c2\u00bf inducida por altas concentraciones de p, en modelos in vitro de calcificaci\u00f3n vascular, podr\u00eda ser un paso necesario para que la cmlv se transforme a c\u00e9lula osteog\u00e9nica.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Calcificaciones vasculares: mecanismos celulares y moleculares<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Calcificaciones vasculares: mecanismos celulares y moleculares <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Addy Rosa Montes De Oca Gonzalez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 C\u00f3rdoba<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 18\/09\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Escolastico Aguilera Tejero<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: rafael Mayer valor <\/li>\n<li>domingo Hernandez marrero (vocal)<\/li>\n<li>v\u00edctor Lorenzo sellares (vocal)<\/li>\n<li>Antonio Canalejo raya (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Addy Rosa Montes De Oca Gonzalez La hiperfosfatemia est\u00e1 estrechamente ligada a las calcificaciones vasculares en la 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