{"id":96472,"date":"2009-08-10T00:00:00","date_gmt":"2009-08-10T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/importancia-de-la-metilacion-y-sumoilacion-de-la-coilina-y-del-factor-de-supervivencia-de-las-motoneuronas-en-el-ensamblaje-del-cuerpo-nuclear-de-cajal\/"},"modified":"2009-08-10T00:00:00","modified_gmt":"2009-08-10T00:00:00","slug":"importancia-de-la-metilacion-y-sumoilacion-de-la-coilina-y-del-factor-de-supervivencia-de-las-motoneuronas-en-el-ensamblaje-del-cuerpo-nuclear-de-cajal","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-celular\/importancia-de-la-metilacion-y-sumoilacion-de-la-coilina-y-del-factor-de-supervivencia-de-las-motoneuronas-en-el-ensamblaje-del-cuerpo-nuclear-de-cajal\/","title":{"rendered":"Importancia de la metilaci\u00f3n y sumoilaci\u00f3n de la coilina y del factor de supervivencia de las motoneuronas en el ensamblaje del cuerpo nuclear de cajal"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Olga Tapia  Martinez <\/strong><\/h2>\n<p>Los cuerpos nucleares de cajal (cbs) son estructuras nucleares implicadas en biog\u00e9nesis de ribonucleoprote\u00ednas nucleares y nucleolares de peque\u00f1o tama\u00f1o (snrnps y snornps) requeridas para el procesamiento nuclear de premrnas y pre-rrnas, respectivamente. El cb concentra la prote\u00edna coilina, un marcador molecular de esta estructura, snrnps, el factor de supervivencia de las neuronas motoras (snm) y las prote\u00ednas que comparte con el nucleolo nopp140 y fibrilarina. Los cb son estructuras dependientes de transcripci\u00f3n, per lo mecanismos de ensemblaje molecular de estos cuerpos son poco conocidos.   en este etudio, se utilizan m\u00e9todos de inmunofluorescencia, expresi\u00f3n ect\u00f3pica de prote\u00ednas del cb  y m\u00e9todos bioqu\u00edmicos para analizar la importancia de dos modificaciones postraduccionales, la metilaci\u00f3n de la coilina y la conjugaci\u00f3n con umo1 del factor smn para el ensamblaje molecular de los cbs. Se ha utilizado la l\u00ednea celular mcf7 como un modelo de hipometilaci\u00f3n end\u00f3gena debido al d\u00e9ficit del gen mtap. La hipometilaci\u00f3n de la coilina conduce al desensamblaje de los cbs y a la relocalizaci\u00f3n nucleolar de la coilina no metilada. Este efecto revierte en c\u00e9lulas transfectadas que expresan el gen mtapwt, indicando que el grado de metilaci\u00f3n de la coilina marca su destino nuclear.  respecto a la importancia de la sumoilaci\u00f3n en el ensamblaje de los cbs, hemos demostrado la existencia  de un subtipo de cbs que concentran sumo1 y la conjugasa de sumo ubc9. En neuronas, hemos detectado la presencia de sumo durante la fase de reformaci\u00f3n de cbs, en la respuesta al estr\u00e9s. Los experimentos de inmunoprecipitaci\u00f3n confirman la interacci\u00f3n de sumo-1 con el factor smn  y demuestran que la lisina d119, portadora de una secuencia consenso de sumoilaci\u00f3n, es esencial para la regulaci\u00f3n del n\u00famero de cbs.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Importancia de la metilaci\u00f3n y sumoilaci\u00f3n de la coilina y del factor de supervivencia de las motoneuronas en el ensamblaje del cuerpo nuclear de cajal<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Importancia de la metilaci\u00f3n y sumoilaci\u00f3n de la coilina y del factor de supervivencia de las motoneuronas en el ensamblaje del cuerpo nuclear de cajal <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Olga Tapia  Martinez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Cantabria<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 08\/10\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Miguel Angel Lafarga Coscojuela<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Mar\u00eda  amor Hurle  gonzalez <\/li>\n<li>piero Crespo baraja (vocal)<\/li>\n<li>pilar Sanchez  testillano (vocal)<\/li>\n<li>josefa Hidalgo   jimenez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Olga Tapia Martinez Los cuerpos nucleares de cajal (cbs) son estructuras nucleares implicadas en biog\u00e9nesis de ribonucleoprote\u00ednas [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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