{"id":96912,"date":"2018-03-11T10:17:16","date_gmt":"2018-03-11T10:17:16","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/la-reconstitucion-en-liposomas-de-trwb-un-nanomotor-que-transporta-dna-revela-la-importancia-del-dominio-transmembrana-en-la-funcion-de-la-proteina\/"},"modified":"2018-03-11T10:17:16","modified_gmt":"2018-03-11T10:17:16","slug":"la-reconstitucion-en-liposomas-de-trwb-un-nanomotor-que-transporta-dna-revela-la-importancia-del-dominio-transmembrana-en-la-funcion-de-la-proteina","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/proteinas\/la-reconstitucion-en-liposomas-de-trwb-un-nanomotor-que-transporta-dna-revela-la-importancia-del-dominio-transmembrana-en-la-funcion-de-la-proteina\/","title":{"rendered":"La reconstitucion en liposomas de trwb, un nanomotor que transporta dna, revela la importancia del dominio transmembrana en la funcion de la proteina"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Ana Julia Vecino Ortega <\/strong><\/h2>\n<p>La prote\u00edna acopladora del pl\u00e1smido conjugativo r388 (trwb), al igual que el resto de los miembros de esta familia, es una prote\u00edna integral de membrana y juega un papel esencial en el proceso de conjugaci\u00f3n de dicho pl\u00e1smido. Este tipo de prote\u00ednas son las encargadas de poner en contacto la maquinaria de procesamiento del dna con la responsable de su transporte, en el fen\u00f3meno de conjugaci\u00f3n bacteriana. Estudios previos revelaron que el dominio transmembrana ejerc\u00eda un papel importante en la estabilidad de la prote\u00edna, de manera que para esclarecer su papel en la actividad de la prote\u00edna, se compar\u00f3 la capacidad de unir nucle\u00f3tidos de trwb con un mutante soluble, trwb\u00c2\u00bfn70. Adem\u00e1s, para elucidar la funci\u00f3n del dominio transmembrana se construyeron  distintos mutantes de delecci\u00f3n de dicho dominio y se realizaron estudios de actividad in vivo, uni\u00f3n a nucle\u00f3tidos y estabilidad. Dado el papel esencial que desempe\u00f1a esta familia de prote\u00ednas en la conjugaci\u00f3n bacteriana y puesto que interaccionan con otras prote\u00ednas de membrana, se procedi\u00f3 a la incorporaci\u00f3n de trwb en liposomas con el fin de analizar su actividad biol\u00f3gica en un sistema m\u00e1s cercano al natural. En este trabajo se estudiaron las condiciones \u00f3ptimas de reconstituci\u00f3n en relaci\u00f3n a la composici\u00f3n lip\u00eddica de los liposomas empleados, relaci\u00f3n l\u00edpido-prote\u00edna, cantidades de detergente empleadas as\u00ed como la orientaci\u00f3n de la prote\u00edna una vez reconstituida. Asimismo, se caracteriz\u00f3 la prote\u00edna reconstituida en liposomas en cuanto a la actividad de uni\u00f3n a nucle\u00f3tidos y se compar\u00f3 su estabilidad con la de prote\u00edna solubilizada en detergente por espectroscopia de infrarrojo.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>La reconstitucion en liposomas de trwb, un nanomotor que transporta dna, revela la importancia del dominio transmembrana en la funcion de la proteina<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 La reconstitucion en liposomas de trwb, un nanomotor que transporta dna, revela la importancia del dominio transmembrana en la funcion de la proteina <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Ana Julia Vecino Ortega <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 30\/10\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Fernando De La Cruz Calahorra<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 Luis Rodriguez arrondo <\/li>\n<li>matxalen Llosa blas (vocal)<\/li>\n<li>german Rivas caballero (vocal)<\/li>\n<li>ismael Mingarro mu\u00f1oz (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Ana Julia Vecino Ortega La prote\u00edna acopladora del pl\u00e1smido conjugativo r388 (trwb), al igual que el resto [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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