{"id":97293,"date":"2018-03-11T10:17:46","date_gmt":"2018-03-11T10:17:46","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/efectos-de-la-leptina-en-el-musculo-esqueletico\/"},"modified":"2018-03-11T10:17:46","modified_gmt":"2018-03-11T10:17:46","slug":"efectos-de-la-leptina-en-el-musculo-esqueletico","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-celular\/efectos-de-la-leptina-en-el-musculo-esqueletico\/","title":{"rendered":"Efectos de la leptina en el m\u00fasculo esquel\u00e9tico"},"content":{"rendered":"

Tesis doctoral de Neira S\u00e1inz Amillo <\/strong><\/h2>\n

A reduced skeletal muscle mass has been observed in leptin-deficient ob\/ob mice. The aim of the present study was to analyze the effect of leptin on the anabolic and catabolic pathways regulating muscle mass. Ten-week-old male wild type (c57bl\/6j) (n=30) and genetically obese ob\/ob mice (c57bl\/6j) (n=30) were divided in control, leptin-treated and pair-fed groups (n=10 per group). The control wild type (wv), pair-fed wild type (wp), control ob\/ob (ov) and pair-fed ob\/ob (op) groups received vehicle (pbs), while leptin-treated wild type (wl) and leptin-treated ob\/ob (ol) groups were intraperitoneally administered with leptin (1 mg\/kg\/d) for 28 days. Muscle mass and fiber size of gastrocnemius, edl and soleus were significantly lower in ov compared to wv (p<0.001), being significantly increased following leptin administration as compared to ov and op groups (p<0.001). These effects were associated with an inactivation of the muscle atrophy-related transcription factor foxo3a (p=0.023), together with a decrease in the protein expression levels of the e3 ubiquitin-ligases mafbx (p=0.014) in gastrocnemius muscle of wild type and ob\/ob mice and of the murf1 as compared to ov and op groups (p<0.05). Protein expression levels of pgc-1\u00c2\u00bf\u00f1, a regulator of muscle fiber type, were lower in ob\/ob mice compared to wild type (p=0.01), being significantly increased by leptin administration (p=0.005). Furthermore, myostatin protein, a negative regulator of muscle growth, was reduced in ol compared to both ov (p=0.026) and op (p=0.015) groups. Leptin administration also activated the positive regulators of cell cycle progression, pcna (p=0.002) and cyclin d1 (p=0.005), in wild type and ob\/ob mice, while it inhibited the negative regulator p27kip1 (p=0.004). In addition, the amount of the slow (p=0.001) and fast (p=0.012) isoforms of the myofibrillar protein troponin t was increased by leptin in wild type and ob\/ob mice. The present study provides evidence that leptin treatment increases muscle mass of ob\/ob mice by inhibiting myofibrillar protein degradation as well as enhancing muscle cell proliferation.\n\n\n\n \n\n\n

Datos acad\u00e9micos de la tesis doctoral \u00abEfectos de la leptina en el m\u00fasculo esquel\u00e9tico<\/strong>\u00ab<\/h3>\n
    \n
  • T\u00edtulo de la tesis:<\/strong>\u00a0 Efectos de la leptina en el m\u00fasculo esquel\u00e9tico <\/li>\n
  • Autor:<\/strong>\u00a0 Neira S\u00e1inz Amillo <\/li>\n
  • Universidad:<\/strong>\u00a0 Navarra<\/li>\n
  • Fecha de lectura de la tesis:<\/strong>\u00a0 20\/11\/2009<\/li>\n<\/ul>\n

     <\/p>\n

    Direcci\u00f3n y tribunal<\/h3>\n
      \n
    • Director de la tesis<\/strong>\n
        \n
      • Gema Fr\u00c1\u00bchbeck Mart\u00ednez<\/li>\n<\/ul>\n<\/li>\n
      • Tribunal<\/strong>\n
          \n
        • Presidente del tribunal: Carlos Dieguez gonzalez <\/li>\n
        • Francisco Javier Salvador rodr\u00edguez (vocal)<\/li>\n
        • Mar\u00eda no Ruiz gayo (vocal)<\/li>\n
        • Mar\u00eda del mar Malagon poyato (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n

           <\/p>\n","protected":false},"excerpt":{"rendered":"

          Tesis doctoral de Neira S\u00e1inz Amillo A reduced skeletal muscle mass has been observed in leptin-deficient ob\/ob mice. The aim […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[849,36483,2135,13253,17749],"tags":[15487,14910,82373,54290,52600,199438],"class_list":["post-97293","post","type-post","status-publish","format-standard","hentry","category-biologia-celular","category-biologia-molecular","category-fisiologia-endocrina","category-fisiologia-muscular","category-navarra","tag-carlos-dieguez-gonzalez","tag-francisco-javier-salvador-rodriguez","tag-gema-frahbeck-Martinez","tag-maria-del-mar-malagon-poyato","tag-maria-no-ruiz-gayo","tag-neira-sainz-amillo"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/97293","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=97293"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/97293\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=97293"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=97293"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=97293"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}