{"id":97756,"date":"2009-04-12T00:00:00","date_gmt":"2009-04-12T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/degradacion-de-compuestos-nitroaromaticos-por-rhodobacter-purificacion-de-la-nitrorreductasa-nprb\/"},"modified":"2009-04-12T00:00:00","modified_gmt":"2009-04-12T00:00:00","slug":"degradacion-de-compuestos-nitroaromaticos-por-rhodobacter-purificacion-de-la-nitrorreductasa-nprb","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/cordoba\/degradacion-de-compuestos-nitroaromaticos-por-rhodobacter-purificacion-de-la-nitrorreductasa-nprb\/","title":{"rendered":"Degradacion de compuestos nitroaromaticos por rhodobacter: purificacion de la nitrorreductasa nprb"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Rodolfo Gomez Cruz <\/strong><\/h2>\n<p>El gen nprb de r. Capsulatus b10s codifica una nitrorreductasa que presenta similitud con miembros de la familia b de las nitrorreductasas insensibles a ox\u00edgeno. Asimismo, nprb tambi\u00e9n muestra cierta similitud, aunque no muy elevada, con la nitrorreductasa npra de r. Capsulatus que ha sido estudiada previamente.\t la prote\u00edna nprb se ha sobreexpresado en c\u00e9lulas de e. Coli fusionada a una cola de polihistidinas, y se ha purificado por cromatograf\u00eda de afinidad. La prote\u00edna recombinante purificada posee actividad con diferentes compuestos nitroarom\u00e1ticos y heteroc\u00edclicos utilizados por otras nitrorreductasas, y puede utilizar como sustrato 2,4,6-trinitrotolueno (tnt), aunque la m\u00e1xima actividad se obtiene con \u00e1cido p\u00edcrico 2,4,6-trinitrofenol. Sin embargo, la prote\u00edna nprb no puede activar eficazmente al prof\u00e1rmaco cb1954 utilizado en terapias antitumorales.  la prote\u00edna purificada contiene fmn y utiliza preferentemente nadh como donador de electrones. Adem\u00e1s, se inhibe por dicumarol y capsaicina, as\u00ed como por cianuro y sulfato de cobre.  la prote\u00edna nprb posee una baja actividad dihidropterina reductasa, a diferencia de npra, pero fisiol\u00f3gicamente podr\u00eda actuar como una nad(p)h-quinona oxidorreductasa. finalmente, se han obtenido varios posibles mutantes por inserci\u00f3n al azar del transpos\u00f3n tn5 que podr\u00edan estar afectados en la ruta degradativa del 2,4-dinitrofenol (dnp) en r. Sphaeroides dsm158s. La caracterizaci\u00f3n de estos mutantes podr\u00e1 permitir dilucidar la ruta degradativa del dnp en esta bacteria, e identificar los principales genes e intermediarios metab\u00f3licos implicados en el proceso.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Degradacion de compuestos nitroaromaticos por rhodobacter: purificacion de la nitrorreductasa nprb<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Degradacion de compuestos nitroaromaticos por rhodobacter: purificacion de la nitrorreductasa nprb <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Rodolfo Gomez Cruz <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 C\u00f3rdoba<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 04\/12\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mar\u00eda  Dolores Roldan Ruiz<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Francisco Castillo rodriguez <\/li>\n<li>amando Garrido pertierra (vocal)<\/li>\n<li>Mar\u00eda Jes\u00fas Delgado ige\u00f1o (vocal)<\/li>\n<li>Mar\u00eda Jos\u00e9 Huertas romera (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Rodolfo Gomez Cruz El gen nprb de r. Capsulatus b10s codifica una nitrorreductasa que presenta similitud con [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36606,973,4578,11152],"tags":[3252,20893,200166,197925,107499,200165],"class_list":["post-97756","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular-de-microorganismos","category-cordoba","category-ingenieria-genetica","category-metabolismo-bacteriano","tag-amando-garrido-pertierra","tag-francisco-castillo-rodriguez","tag-maria-dolores-roldan-ruiz","tag-maria-jesus-delgado-igeno","tag-maria-jose-huertas-romera","tag-rodolfo-gomez-cruz"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/97756","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=97756"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/97756\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=97756"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=97756"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=97756"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}