{"id":98332,"date":"2018-03-11T10:19:08","date_gmt":"2018-03-11T10:19:08","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/modulacion-de-la-estructura-y-funcion-de-la-rnasa-a-y-de-presuntas-ribonucleasas-prebioticas\/"},"modified":"2018-03-11T10:19:08","modified_gmt":"2018-03-11T10:19:08","slug":"modulacion-de-la-estructura-y-funcion-de-la-rnasa-a-y-de-presuntas-ribonucleasas-prebioticas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/modulacion-de-la-estructura-y-funcion-de-la-rnasa-a-y-de-presuntas-ribonucleasas-prebioticas\/","title":{"rendered":"Modulaci\u00f3n de la estructura y funci\u00f3n de la rnasa a y de presuntas ribonucleasas prebi\u00f3ticas."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jorge  Pedro Lopez Alonso <\/strong><\/h2>\n<p>\u00edndice 1. Introducci\u00f3n general 1.1. Introducci\u00f3n 11 1.2. Objetivos generales 13 1.3. Bibliograf\u00eda 15 2. Material y m\u00e9todos 2.1. Estructura de prote\u00ednas 19 2.2. Estudio de prote\u00ednas mediante rmn 23 2.2.1. Espectroscop\u00eda bidimensional 24 2.2.2. Espectroscop\u00eda tridimensional 25 2.2.3. Experimentos hsqc editados en c\u00edY y en c\u00c2\u00bf 27 2.2.5. Experimentos de intercambio prot\u00f3n \/ deuterio 29 2.2.6. C\u00e1lculo de estructura de prote\u00ednas 30 2.3. Otras t\u00e9cnicas utilizadas para el estudio de prote\u00ednas 31 2.3.1. Dicro\u00edsmo circular 31 2.3.2. Cromatograf\u00eda l\u00edquida de prote\u00ednas (fplc) 32 2.3.3. Electroforesis en gel de poliacrilamida 33 2.4. La ribonucleasa a 34 2.4.1. La ribonucleasa s 36 2.4.2. Oligomerizaci\u00f3n de la ribonucleasa a 37 2.4.3. Medida de la actividad de ribonucleasas frente a arn 39 2.5. Estructura de \u00e1cidos nucleicos 41 2.6. Bibliograf\u00eda 44 3. La oligomerizaci\u00f3n de la rnasa a 3.1. Introducci\u00f3n 51 3.1.1. Los amiloides 51 3.1.2. La oligomerizaci\u00f3n de la rnasa a 57 3.2. La oligomerizaci\u00f3n de la rnasa s 59 3.2.1. Obtenci\u00f3n de olig\u00f3meros 59 3.2.2. Caracterizaci\u00f3n estructural del d\u00edmero de la rnasa s 65 3.2.3. Disociaci\u00f3n del d\u00edmero de la rnasa s 70 3.3. Mecanismo de formaci\u00f3n de los olig\u00f3meros de la rnasa a 74 3.3.1. Caracterizaci\u00f3n mediante espectroscop\u00eda uv y dicro\u00edsmo circular 74 3.3.2. Caracterizaci\u00f3n mediante rmn 77 3.3.3. Caracterizaci\u00f3n de p\u00e9ptidos de la rnasa a en 40% de \u00e1cido ac\u00e9tico 87 3.3.4. Dependencia de la concentraci\u00f3n en la formaci\u00f3n de olig\u00f3meros 90 3.3.5. Posible mecanismo de oligomerizaci\u00f3n de la rnasa a 91 3.3.6. Cin\u00e9tica de plegamiento y desplegamiento de la rnasa a 95 3.4. Bibliograf\u00eda 98 \u00edndice 8 4. Hacia ribonucleasas m\u00e1s eficaces y espec\u00edficas 4.1. Introducci\u00f3n 108 4.1.1. Las ribonucleasas como agentes antitumorales 108 4.1.2. Ribonucleasas semisint\u00e9ticas 111 4.2. Modulando la especificidad: conjugados rnasa-oligonucle\u00f3tido 114 4.2.1. Formaci\u00f3n de conjugados rnasa-adn y rnasa-pna 114 4.2.2. Caracterizaci\u00f3n de los conjugados (dt)nrnasa mediante rmn 115 4.2.3. Actividad de los conjugados de rnasa a 117 4.3. Aumentando la eficacia: estabilizaci\u00f3n del c-d\u00edmero de la rnasa 128 4.3.1. Elecci\u00f3n del edc como agente de entrecruzamiento 128 4.3.2. Posibles grupos diana de la reacci\u00f3n 132 4.3.3. Reacci\u00f3n del c-d\u00edmero de la rnasa a con edc 134 4.3.4. Aislamiento y caracterizaci\u00f3n de los productos 135 4.3.5. Actividad ribonucleol\u00edtica 145 4.4. Bibliograf\u00eda 149 5. Kia7h: una presunta ribonucleasa prebi\u00f3tica 5.1. Introducci\u00f3n 156 5.1.1. El origen de la vida 156 5.1.2. Los p\u00e9ptidos kia7 161 5.2. Estructura de los p\u00e9ptidos kia7 162 5.2.1. Plegamiento y estabilidad conformacional 162 5.2.2. Estructura terciaria y cuaternaria de kia7h y kia7w 167 5.3. Actividad de los p\u00e9ptidos kia7 171 5.3.1. Actividad frente a una horquilla de arn 171 5.3.2. Actividad frente a un arn de transferencia 180 5.3.3. Actividad frente a un arn que no forma horquillas 182 5.3.4. Posible mecanismo de corte 184 5.4. Implicaciones para la vida primitiva 187 5.5. Bibliograf\u00eda 188<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Modulaci\u00f3n de la estructura y funci\u00f3n de la rnasa a y de presuntas ribonucleasas prebi\u00f3ticas.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Modulaci\u00f3n de la estructura y funci\u00f3n de la rnasa a y de presuntas ribonucleasas prebi\u00f3ticas. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jorge  Pedro Lopez Alonso <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 18\/12\/2009<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Carlos Gonz\u00e1lez Ib\u00e1\u00f1ez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: enrique Pedroso muller <\/li>\n<li>subramanian Padmanabhan (vocal)<\/li>\n<li>vicente March\u00e1n sancho (vocal)<\/li>\n<li>ramon Campos olivas (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jorge Pedro Lopez Alonso \u00edndice 1. Introducci\u00f3n general 1.1. Introducci\u00f3n 11 1.2. Objetivos generales 13 1.3. Bibliograf\u00eda [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[199,17],"tags":[78595,1101,201141,201143,201142,183220],"class_list":["post-98332","post","type-post","status-publish","format-standard","hentry","category-fisica","category-quimica","tag-carlos-gonzalez-ibanez","tag-enrique-pedroso-muller","tag-jorge-pedro-lopez-alonso","tag-ramon-campos-olivas","tag-subramanian-padmanabhan","tag-vicente-marchan-sancho"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/98332","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=98332"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/98332\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=98332"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=98332"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=98332"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}