{"id":98643,"date":"2018-03-11T10:19:30","date_gmt":"2018-03-11T10:19:30","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/development-of-cellullar-microarrays-for-stem-cell-culture-and-early-stage-differentiation-evaluation\/"},"modified":"2018-03-11T10:19:30","modified_gmt":"2018-03-11T10:19:30","slug":"development-of-cellullar-microarrays-for-stem-cell-culture-and-early-stage-differentiation-evaluation","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-celular\/development-of-cellullar-microarrays-for-stem-cell-culture-and-early-stage-differentiation-evaluation\/","title":{"rendered":"Development of cellullar microarrays for stem cell culture and early stage differentiation evaluation"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Santiago Andr\u00e9s Rodr\u00edguez Segu\u00ed <\/strong><\/h2>\n<p>El desarrollo de microambientales artificiales ordenados en un formato de microarray (microarrays celulares, tambi\u00e9n conocidos en ingl\u00e9s como \u00abcellular microarrays\u00bb) se ha propuesto como una herramienta que permite el an\u00e1lisis a gran escala de combinaciones de factores que afectan la diferenciaci\u00f3n de c\u00e9lulas madre. Este novedoso acercamiento puede implementarse para imitar superf\u00edcies planas y de esta forma estudiar su diferenciaci\u00f3n, este fin tiene directas implicaciones en el desarrollo de nuevos biomateriales y en ingenier\u00eda de tejidos.   el objetivo general del presente trabajo de tesis consisti\u00f3 en el dise\u00f1o y evaluaci\u00f3n de microarrays celulares construidos con c\u00e9lulas madre con el objetivo de estudiar su diferenciaci\u00f3n hacia osteoblastos (c\u00e9lulas \u00f3seas) en funci\u00f3n de los factores impresos. A estos fines, se utiliz\u00f3 una trazadora de microarrays (microarray plotter) para realizar los microarrays donde diversos factores inmovilizados en micro islas impresas sobre substratos activados q\u00fa\u00edmicamente (vidrio y polimetilmetacrilato (pmma), entre otros). Sobre estos substratos impresos se cultivaron c\u00e9lulas por per\u00edodos de 1 a 8 d\u00edas y se evalu\u00f3 su diferenciaci\u00f3n al cabo de este tiempo de cultivo.   en la presente tesis se hizo una comparaci\u00f3n de substratos en funci\u00f3n de la cantidad de prote\u00edna impresa que retienen inmovilizada en su superf\u00edcie. Otro objetivo consisti\u00f3 en optimizar los par\u00e1metros para la formaci\u00f3n del microarray celular ( tales como tiempo y concentraci\u00f3n de sembrado de c\u00e9lulas, medio de cultivo, etc..). Finalmente se evalu\u00f3 la diferenciqaci\u00f3n de una l\u00ednea celular hacia osteoblastos en funci\u00f3n de la presencia o no d eun factor de crecimiento inmovilizado en las islas de microarray.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Development of cellullar microarrays for stem cell culture and early stage differentiation evaluation<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Development of cellullar microarrays for stem cell culture and early stage differentiation evaluation <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Santiago Andr\u00e9s Rodr\u00edguez Segu\u00ed <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 15\/01\/2010<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Josep Samitier Marti<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: francesc Cardellach l\u00f3pez <\/li>\n<li>albert Cornet i calveras (vocal)<\/li>\n<li>  (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Santiago Andr\u00e9s Rodr\u00edguez Segu\u00ed El desarrollo de microambientales artificiales ordenados en un formato de microarray (microarrays celulares, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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