{"id":99148,"date":"2010-12-02T00:00:00","date_gmt":"2010-12-02T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/identificacion-de-nuevos-mecanismos-de-virulencia-en-fusarium-oxysporum-mediante-mutagenesis-por-insercion-de-un-transposon\/"},"modified":"2010-12-02T00:00:00","modified_gmt":"2010-12-02T00:00:00","slug":"identificacion-de-nuevos-mecanismos-de-virulencia-en-fusarium-oxysporum-mediante-mutagenesis-por-insercion-de-un-transposon","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/cordoba\/identificacion-de-nuevos-mecanismos-de-virulencia-en-fusarium-oxysporum-mediante-mutagenesis-por-insercion-de-un-transposon\/","title":{"rendered":"Identificacion de nuevos mecanismos de virulencia en fusarium oxysporum mediante mutagenesis por insercion de un transposon"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Manuel Sanchez Lopez-berges <\/strong><\/h2>\n<p>Fusarium oxysporum es un hongo fitopat\u00f3geno considerado el principal agente causante de la marchitez vascular. Esta enfermedad provoca importantes p\u00e9rdidas en una gran variedad de cultivos ya que la especie se encuentra distribuida en la mayor\u00eda de suelos cultivados. F. Oxysporum es tambi\u00e9n un emergente pat\u00f3geno oportunista de humanos, como refleja el aumento de casos de pacientes inmunodeprimidos con infecciones sist\u00e9micas que, en muchas ocasiones, son mortales. Muchos de los mecanismos utilizados por los hongos para infectar a sus hu\u00e9spedes son a\u00fan desconocidos. Este trabajo se ha centrado en el descubrimiento de nuevos factores de virulencia utilizando un abordaje de gen\u00e9tica directa. Para conseguir el objetivo, se ha obtenido una colecci\u00f3n de mutantes por inserci\u00f3n mediante transposones que ha sido sometida a un escrutinio para identificar mutantes afectados en virulencia. Gracias a este escrutinio, hemos establecido dos l\u00edneas de investigaci\u00f3n independientes y novedosas directamente relacionadas con la patogenicidad del hongo:  1.La familia de prote\u00ednas velvet, implicada en el control del desarrollo y el metabolismo secundario, es tambi\u00e9n importante en la patog\u00e9nesis. Las mutaciones en dos de los genes de la familia, vea o velb, reducen considerablemente la capacidad infectiva de f. Oxysporum. 2.La segunda l\u00ednea de investigaci\u00f3n se ha centrado en la conexi\u00f3n entre el metabolismo del nitr\u00f3geno y la virulencia. Hemos demostrado que el amonio inhibe funciones de virulencia como el crecimiento invasivo o la adhesi\u00f3n al hospedador derivando en una reducci\u00f3n de los s\u00edntomas de la enfermedad vascular en presencia de esta fuente de nitr\u00f3geno. Esta inhibici\u00f3n de las funciones de virulencia activada por amonio est\u00e1 mediada por la prote\u00edna kinasa tor y el factor de trasncripci\u00f3n meab.     fusarium oxysporum is a plant pathogen considered the principal agent of the vascular wilt disease, affecting a large variety of economically important crops worldwide. F. Oxysporum is known as a serious emerging pathogen of humans due to the increasing number of severe cases reported and to its broad resistance to the available antifungal drugs. Fusarium now represents the second most frequent mold causing invasive fungal infections in immunocompromised patients, frequently with lethal outcomes. Many of the fungal mechanisms used for host colonization are largely unknown. This work has focused in the identification of new virulence factors using a forward genetic approach. To rich the aim, a transposon tagged mutant collection was isolated and screened for virulence. Thanks to the virulence screening we established two independent and original lines of investigation directly related with the fungal pathogenesis:  1.Velvet, a protein family implicated in development and secondary metabolism regulation, is also important for pathogenicity. Mutations in vea or velb, two genes of the velvet family, considerably affect virulence in f. Oxysporum. 2.The second line has focused in the connection between nitrogen metabolism and virulence. We proved that the preferred nitrogen source ammonium inhibits many virulence functions such us invasive growth or host adhesion, provoking a very significant delay in the vascular wilt symptoms. Nitrogen signaling is mediated by the protein kinase tor and the transcription factor meab.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Identificacion de nuevos mecanismos de virulencia en fusarium oxysporum mediante mutagenesis por insercion de un transposon<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Identificacion de nuevos mecanismos de virulencia en fusarium oxysporum mediante mutagenesis por insercion de un transposon <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Manuel Sanchez Lopez-berges <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 C\u00f3rdoba<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 12\/02\/2010<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Concepcion Hera  Diaz De Lia\u00f1o<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Mar\u00eda  isabel Gonzalez roncero <\/li>\n<li>Jos\u00e9 Mar\u00eda D\u00edaz m\u00ednguez (vocal)<\/li>\n<li>eduardo Antonio Espeso fernandez (vocal)<\/li>\n<li>Jos\u00e9 Luis Crespo gonz\u00e1lez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Manuel Sanchez Lopez-berges Fusarium oxysporum es un hongo fitopat\u00f3geno considerado el principal agente causante de la marchitez 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