{"id":99402,"date":"2018-03-11T10:20:34","date_gmt":"2018-03-11T10:20:34","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-estructural-de-complejos-implicados-en-interacciones-con-el-adn\/"},"modified":"2018-03-11T10:20:34","modified_gmt":"2018-03-11T10:20:34","slug":"analisis-estructural-de-complejos-implicados-en-interacciones-con-el-adn","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/analisis-estructural-de-complejos-implicados-en-interacciones-con-el-adn\/","title":{"rendered":"An\u00e1lisis estructural de complejos implicados en interacciones con el adn"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Javier Coloma Cervera <\/strong><\/h2>\n<p>La interacci\u00f3n entre las prote\u00ednas y el adn es esencial para distintos procesos celulares. Uno de estos procesos es la replicaci\u00f3n del adn cromos\u00f3mico donde participan numerosos complejos de prote\u00ednas. Uno de ellos es el heterotetr\u00e1mero gins que junto a la prote\u00edna cdc45 y el complejo mcm2-7 forma el cmg, implicado en el desenrrollamiento del adn. Hemos purificado el complejo gins humano (hgins) y hemos mostrado que se une a adn de cadena sencilla. Usando microscop\u00eda electr\u00f3nica de part\u00edculas individuales y reconstrucci\u00f3n 3d obtuvimos una estructura a baja resoluci\u00f3n del complejo hgins, que muestra forma de herradura. La espectrometr\u00eda de masas y la localizaci\u00f3n por un anticuerpo monoclonal de una subunidad del tetr\u00e1mero revel\u00f3 la organizaci\u00f3n de las subunidades dentro del complejo hgins. El an\u00e1lisis detallado del volumen obtenido junto a la comparaci\u00f3n con las estructuras at\u00f3micas disponibles sugieren la presencia de al menos dos conformaciones del tetr\u00e1mero. La estructura y el modo de uni\u00f3n a adn sugieren un posible papel  de gins en el contexto del complejo cmg. las meganucleasas se est\u00e1n convirtiendo en poderosas herramientas para la ingenier\u00eda de genomas y terapia g\u00e9nica. Se han modificado la interacci\u00f3n de la meganucleasa i-crei con el adn de modo que reconozca secuencias espec\u00edficas del gen humano rag1. Se presentan dos estructuras cristalogr\u00e1ficas de un mutante heterodim\u00e9rico de i-crei con un nuevo dise\u00f1o de cadena \u00fanica, scv3-v2(g19s), en el que se han unido dos mon\u00f3meros en un polip\u00e9ptido. Este mutante reconoce un fragmento del gen humano rag1 in vivo e in vitro. Las dos estructuras de scv3-v2(g19s) se han obtenido en presencia de dos cationes distintos: manganeso, que permite que se produzca la reacci\u00f3n de corte del adn de doble cadena, y calcio, en cuya presencia el gen rag1 no est\u00e1 cortado.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>An\u00e1lisis estructural de complejos implicados en interacciones con el adn<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 An\u00e1lisis estructural de complejos implicados en interacciones con el adn <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Javier Coloma Cervera <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 25\/02\/2010<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Guillermo Montoya Blanco<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 Mar\u00eda Valpuesta moralejo <\/li>\n<li>carmen San mart\u00edn pastrana (vocal)<\/li>\n<li>Juan Hermoso dominguez (vocal)<\/li>\n<li>Luis Blanco d\u00e1vila (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Javier Coloma Cervera La interacci\u00f3n entre las prote\u00ednas y el adn es esencial para distintos procesos celulares. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36483,115],"tags":[149657,70225,202883,49522,67452,2617],"class_list":["post-99402","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular","category-bioquimica-molecular","tag-carmen-san-martin-pastrana","tag-guillermo-montoya-blanco","tag-javier-coloma-cervera","tag-jose-maria-valpuesta-moralejo","tag-juan-hermoso-dominguez","tag-luis-blanco-davila"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/99402","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=99402"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/99402\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=99402"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=99402"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=99402"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}