{"id":99480,"date":"2010-01-03T00:00:00","date_gmt":"2010-01-03T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/structural-and-functional-analysis-of-the-dna-packaging-protein-ul89-from-human-cytomegalovirus\/"},"modified":"2010-01-03T00:00:00","modified_gmt":"2010-01-03T00:00:00","slug":"structural-and-functional-analysis-of-the-dna-packaging-protein-ul89-from-human-cytomegalovirus","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/structural-and-functional-analysis-of-the-dna-packaging-protein-ul89-from-human-cytomegalovirus\/","title":{"rendered":"Structural and functional analysis of the dna packaging protein ul89 from human cytomegalovirus"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Marta Nadal Rovira <\/strong><\/h2>\n<p>Human cytomegalovirus (hcmv) is one of the eight human herpesvirus. Its infection rarely produces significant consequences in people with a competent immune system but can cause serious life threatening complications in immunocompromised people.   during viral replication, hcmv packages its dna into the procapsid via the terminase complex that includes the ul89 protein. This complex nicks the newly-synthesized long dna concatamers into genome units, suitable for encapsidation. Despite its high interest, no structural information is available for such herpesvirus encapsidation system.  this phd dissertation covers the work done for the structural and functional analysis of the hcmv ul89 protein; from the gene cloning to the structure discussion.  producing soluble and pure recombinant protein is a bottleneck for many interesting projects, as it was for ul89. With the goal of obtaining soluble full-length ul89, numerous strategies tried were unsuccessfully. However, using a new high throughput combinatory method, esprit, we found a soluble domain of ul89. We could crystallize this construct and solve its three-dimensional structure at resolution of 2.15 \u00ed\u00a5.  Finally, we performed activity assays to characterise its function.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Structural and functional analysis of the dna packaging protein ul89 from human cytomegalovirus<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Structural and functional analysis of the dna packaging protein ul89 from human cytomegalovirus <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Marta Nadal Rovira <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/03\/2010<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mar\u00eda Sol? Vilarrubias<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ignacio Fita rodriguez <\/li>\n<li>darren Hart (vocal)<\/li>\n<li>  (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Marta Nadal Rovira Human cytomegalovirus (hcmv) is one of the eight human herpesvirus. Its infection rarely produces [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[203001,15733,203000,202999],"class_list":["post-99480","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-darren-hart","tag-ignacio-fita-rodriguez","tag-maria-sol-vilarrubias","tag-marta-nadal-rovira"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/99480","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=99480"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/99480\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=99480"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=99480"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=99480"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}