Role of homeobox nkx2-3 protein in marginal-zone b-cell lymhpomagenesis using an in vivo mouse model

Tesis doctoral de María Mena Varas

Nkx2 homeobox family proteins play a role in cancer development. Molecular cloning of a translocation t(10;14)(q24;q32) from a marginal-zone b-cell lymphoma revealed nkx2-3 as an igh partner gene, leading to increased nkx2-3 expression with respect to b lymphocytes. Nkx2-3 overexpression was also detected in tumor cells from a subset of patients with extranodal and splenic marginal-zone lymphomas, but not with other mature b-cell malignancies. While nkx2-3 deficient mice exhibited atrophic spleens with absence of marginal-zone b cells, transgenic mice with expression of nkx2-3 in b cells showed progressive splenomegaly with marginal-zone expansion, and eventually developed tumors faithfully recapitulating the phenotype, cellular and molecular biology of human marginal-zone lymphomas. Mechanistically, nkx2-3 induced constitutive b-cell receptor (bcr) signaling by phosphorylating lyn and syk kinases. These molecules enhanced proliferation and eventually acquiring genomic rearrangements that triggered nf-κb and pi3k-akt pathways to drive malignant transformation. This study implicates oncogenic nkx2-3 in marginal-zone lymphomagenesis, and provides a valid experimental mouse model for studying the biology and therapy of human lymphoma.

 

Datos académicos de la tesis doctoral «Role of homeobox nkx2-3 protein in marginal-zone b-cell lymhpomagenesis using an in vivo mouse model«

  • Título de la tesis:  Role of homeobox nkx2-3 protein in marginal-zone b-cell lymhpomagenesis using an in vivo mouse model
  • Autor:  María Mena Varas
  • Universidad:  Navarra
  • Fecha de lectura de la tesis:  21/09/2015

 

Dirección y tribunal

  • Director de la tesis
    • José ángel Martínez Climent
  • Tribunal
    • Presidente del tribunal: María dolores Odero de dios
    • manuela Mollejo villanueva (vocal)
    • césar Cobaleda hernández (vocal)
    • ignacio Pérez roger (vocal)

 

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