Tesis doctoral de Gabriela Valls Sierra
A recent role for rac1 gtpase in canonical wnt signaling was recently demonstrated, being required for íY-catenin translocation to the nucleus. We find that rac1 is stimulated in sw-480 or hek-293 cells upon wnt3a addition. This up-regulation temporally correlates with p120-catenin direct binding to rac1 and vav2, an association that happens after the release of p120-catenin from e-cadherin. P120-catenin and vav2 are required for rac1 stimulation by wnt3a, and for íY-catenin translocation to the nucleus. The interaction of rac1 and vav2 with p120-catenin increases upon serine/threonine phosphorylation by ck1 and tyrosine dephosphorylation of p120-catenin, two modifications stimulated by wnt3a. When over-expressed, p120-catenin mutants unable to bind rac1 or vav2 fail to stimulate rac1 activity. Moreover, since p120-catenin depletion disrupts gastrulation in xenopus, we analyzed p120-catenin mutants for their ability to rescue this phenotype. In contrast to the wild-type protein or other controls, p120-catenin point mutants unable to bind rac1 or vav2, failed to rescue p120 depletion. Collectively, these results indicate that p120-catenin is required for rac1 activation upon wnt signaling, through binding to vav2 and rac1 proteins.
Datos académicos de la tesis doctoral «P120-catenin and rac1: key players in canonical wnt signaling«
- Título de la tesis: P120-catenin and rac1: key players in canonical wnt signaling
- Autor: Gabriela Valls Sierra
- Universidad: Autónoma de barcelona
- Fecha de lectura de la tesis: 30/09/2011
Dirección y tribunal
- Director de la tesis
- Mireia Duñach MasJuan
- Tribunal
- Presidente del tribunal: francesc Vinyals canals
- salvador Aznar benitah (vocal)
- (vocal)
- (vocal)